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pT358/pS360-GPR132 (phospho-GPR132 Antibody)

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Agonist-induced Threonine358/Serine360 phosphorylation of GPR132
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  • 7TM0128C
  • 100 µl
  • Rabbit
Threonine358/Serine360 (T358/S360) is major phosphorylation site of human GPR132 receptor. The... more

Threonine358/Serine360 (T358/S360) is major phosphorylation site of human GPR132 receptor. The pT358/pS360-GPR132 antibody detects phosphorylation in response to agonists. T358/S360 phosphorylation is likely to be involved in efficient ligand sequestration by GPR132.

  Alternative Names G Protein-coupled Receptor G2A IUPHAR Target ID... more

 

Alternative Names G Protein-coupled Receptor G2A
IUPHAR Target ID  128
UniProt ID  Q9UNW8
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human GPR132 around the phosphorylation site of Thr358/Ser360.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine358/Serine360 phosphorylation of GPR132.   Upper... more

Figure 1. Agonist-induced Threonine358/Serine360 phosphorylation of GPR132. Upper panel, HEK293 cells transiently expressing the human GPR132-Receptor were either not exposed (vehicle) or exposed to 100 nM of specific GPR132-Receptor agonist 9-HODE for 5 minutes. Cells were lysed and immunoblotted with the anti-pT358/pS360-GPR132 antibody (7TM0128C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-GRP132 antibody to confirm equal loading of the gel.

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