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HA-Tag, Anti-HA Epitope Tag Antibody

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  • 7TM000HA
  • 100 µl
  • Rabbit
The anti-HA tag antibody is directed against amino acids 98-106 of the human influenza... more

The anti-HA tag antibody is directed against amino acids 98-106 of the human influenza hemagglutinin (HA). The HA tag can used as a general epitope tag in expression vectors. The anti-HA antibody can be used to detect total receptors in Western blots independent of phosphorylation. The anti-HA tag antibody can also be used to isolate and enrich receptors from tissue lysates. It also detects HA-tagged receptors in cultured cells and tissue sections by immunohistochemistry.

  Alternative Names  - IUPHAR Target ID  - UniProt... more

 

Alternative Names  -
IUPHAR Target ID  -
UniProt ID  -
Western Blot (WB)  1:1000
Immunocytochemistry (ICC) 1:200
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide presents amino acids 98-106 of the human influenza hemagglutinin (HA)
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Validation of HA-tagged µ-Opioid Receptor in stable HEK293 cell lines. Native... more

Figure 1. Validation of HA-tagged µ-Opioid Receptor in stable HEK293 cell lines. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the HA-tagged µ-Opioid Receptor (HA-MOP) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.

Figure 2. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged Serotonin Receptor 4 (HA-5-HT4), HA-tagged Serotonin Receptor 6 (HA-5-HT6), HA-tagged Serotonin Receptor 2A (HA-5-HT2A), HA-tagged Cholecystokinin Receptor 1  (HA-CCK1) or HA-tagged Cholecystokinin Receptor 2  (HA-CCK2) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.

Figure 3. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged Adenosin Diphosphate Receptor 4 (HA-P2Y4), HA-tagged Adenosin Diphosphate Receptor 12 (HA-P2Y12), HA-tagged Alpha-1 Adrenergic Receptor A (HA-α1A), HA-tagged Dopamine Receptor 5 (HA-D5) or HA-tagged Histamine H2 receptor (HA-H2) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.

Figure 4. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged Hypocretin Receptor 1/Orexin Receptor 1 (HA-OX1), HA-tagged Canabinoid Receptor 1 (HA-CB1), HA-tagged Canabinoid Receptor 1 (HA-CB2), HA-tagged Galanin Receptor 1  (HA-GAL1) or HA-tagged G Protein-coupled Estrogen Receptor  (HA-GPER) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.

Figure 5. Immuncytochemical verification of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged metabotropic Glutamate Receptor 2 (HA-mGLU2), HA-tagged Calcitonin Receptor-like Receptor (HA-CALCRL), HA-tagged Corticotropin-releasing Factor Receptor 1 (HA-CRF1), HA-tagged orphan G Protein-coupled Receptor 20 (HA-GPR20), metabotropic Glutamate Receptor 4 (HA-mGLU4) or HA-tagged Dopamine receptor 1 (HA-D1) were immunocytochemically stained with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:200. Note, the receptors are located at the plasma membrane of the cells.

Figure 6. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged C-C chemokine receptor 5 (HA-CCR5), HA-tagged C-C chemokine receptor 7 (HA-CCR7), HA-tagged C-C chemokine receptor 8 (HA-CCR8), HA-tagged C-C chemokine receptor 9 (HA-CCR9), HA-tagged C-C chemokine receptor 10 (HA-CCR10), HA-tagged muscarinic acetylcholine receptor 3 (HA-M3), HA-tagged histamine H1 receptor (HA-H1), HA-tagged C-X3-C motif chemokine receptor 1 (HA-CX3CR1) or HA-tagged hypocretin receptor 1/orexin receptor 1 (HA-OX1) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.

Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll... more

Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOP receptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists. Sci Signal. 2019 Mar 26;12(574). pii: eaau8072. doi: 10.1126/scisignal.aau8072. PubMed PMID: 30914485; PubMed Central PMCID: PMC6934085.

Miess E, Gondin AB, Yousuf A, Steinborn R, Mösslein N, Yang Y, Göldner M, Ruland JG, Bünemann M, Krasel C, Christie MJ, Halls ML, Schulz S, Canals M. Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization. Sci Signal. 2018 Jul 17;11(539). pii: eaas9609. doi: 10.1126/scisignal.aas9609. PubMed PMID: 30018083.

Petrich A, Mann A, Kliewer A, Nagel F, Strigli A, Märtens JC, Pöll F, Schulz S. Phosphorylation of threonine 333 regulates trafficking of the human sst5 somatostatin receptor. Mol Endocrinol. 2013 Apr;27(4):671-82. doi: 10.1210/me.2012-1329. Epub 2013 Feb 15. PubMed PMID: 23418396; PubMed Central PMCID: PMC5416807.

Pöll F, Doll C, Schulz S. Rapid dephosphorylation of G protein-coupled receptors by protein phosphatase 1β is required for termination of β-arrestin-dependent signaling. J Biol Chem. 2011 Sep 23;286(38):32931-6. doi: 10.1074/jbc.M111.224899. Epub 2011 Jul 27. PubMed PMID: 21795688; PubMed Central PMCID: PMC3190940.

Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049. PubMed PMID: 30726732.

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