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GPR84 (IHC-grade), G Protein-Coupled Receptor 84 Antibody

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  • 7TM0120N-IC
  • 100 µl
  • Rabbit
The GPR84 antibody is directed against the distal end of the carboxyl-terminal tail of human... more

The GPR84 antibody is directed against the distal end of the carboxyl-terminal tail of human GRP84. It can be used to detect total GPR84 receptors in Western blots independent of phosphorylation. The GPR84 antibody can also be used to isolate and enrich GPR84 receptors from cell and tissue lysates. It also detects GPR84 in cultured cells and tissue sections by immunohistochemistry.

  Alternative Names GPR84, G Protein-coupled Receptor 84 IUPHAR Target... more

 

Alternative Names GPR84, G Protein-coupled Receptor 84
IUPHAR Target ID  120
UniProt ID  Q9NQS5
Western Blot (WB)  1:1000
Immunohistochemistry (IHC) 1:100
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide presents distal end of carboxyl-terminal tail of human GPR84
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Immunohistochemical identification of G Protein-coupled Receptor 84 in lymph node.... more

Figure 1. Immunohistochemical identification of G Protein-coupled Receptor 84 in lymph node. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR84 (G Protein-coupled Receptor 84) antibody (7TM0120N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR84 receptors were detected at the plasma membrane of cells in lymph node.

Figure 2. Immunohistochemical identification of G Protein-coupled Receptor 84 in cerebral cortex. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR84 (G Protein-coupled Receptor 84) antibody (7TM0120N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR84 receptors were detected at the plasma membrane of cells in cerebral cortex.

Figure 3. Validation of the G Protein-coupled Receptor 84 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the G Protein-coupled Receptor 84 (GPR84) were lysed and immunoblotted with the anti-GPR84 antibody (7TM0120N-IC) at a dilution of 1:1000.

Marsango S, Ward RJ, Jenkins L, Butcher AJ, Al Mahmud Z, Dwomoh L, Nagel F, Schulz S, Tikhonova... more

Marsango S, Ward RJ, Jenkins L, Butcher AJ, Al Mahmud Z, Dwomoh L, Nagel F, Schulz S, Tikhonova IG, Tobin AB, Milligan G. Selective phosphorylation of threonine residues defines GPR84-arrestin interactions of biased ligands. J Biol Chem. 2022 May;298(5):101932. doi: 10.1016/j.jbc.2022.101932. Epub 2022 Apr 12. PMID: 35427647; PMCID: PMC9118924.

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