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pS336/pS337-CCR5 (phospho-Chemokine Receptor 5 Antibody)

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  • 7TM0062A
  • 100 µl
  • Rabbit
Serine336/Serine337 (S336/S337) is a major phosphorylation site of the CCR5 receptor.... more

Serine336/Serine337 (S336/S337) is a major phosphorylation site of the CCR5 receptor. The pS336/pS337-CCR5 antibody detects phosphorylation in response to high- and low-efficacy agonists and after PKC activation. S336/S337 phosphorylation is a key regulator of CCR5 desensitization, β-arrestin recruitment and internalization.

  Alternative Names CCR5, CKR5, Chemokine Receptor 5, Chemokine (C-C motif)... more

 

Alternative Names CCR5, CKR5, Chemokine Receptor 5, Chemokine (C-C motif) Receptor 5, MIP-1 alpha Receptor
IUPHAR Target ID  62
UniProt ID  P51681
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  -
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human CCR5 around the phosphorylation site of Ser336/Ser337
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine336/Serine337 phosphorylation of the Chemokine Receptor 5.... more

Figure 1. Agonist-induced Serine336/Serine337 phosphorylation of the Chemokine Receptor 5. Upper panel, HEK293 cells stably expressing the Chemokine Receptor 5 (CCR5) were either not exposed or exposed to 100 nM specific Chemokine receptor 5 agonist CCL5 or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pS336/pS337-CCR5 antibody (7TM0062A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CCR5 antibody to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Chemokine Receptor 5 phosphorylation using three phosphosite-specific antibodies. Upper three panels, HEK293 cells stably expressing the Chemokine Receptor 5 (CCR5) were either not exposed or exposed to increasing concentrations of specific Chemokine receptor 5 agonist CCL5 ranging from 3 nM to 100 nM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS336/pS337-CCR5 antibody (7TM0062A) or pT340-CCR5 antibody (7TM0062B) or pS342-CCR5 antibody (7TM0062C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CCR5 antibody to confirm equal loading of the gel.


Be the first to decipher the CCR5 phosphorylation barcode and let us know. more

Be the first to decipher the CCR5 phosphorylation barcode and let us know.

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