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pS332/pS334-C5a1 (phospho-Complement C5a Receptor 1 Antibody)

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  • 7TM0032B
  • 100 µl
  • Rabbit
Serine332/serine334 (S332/S334) is a major phosphorylation site of the C5a1 receptor. The... more

Serine332/serine334 (S332/S334) is a major phosphorylation site of the C5a1 receptor. The pS332/pS334-C5a1 antibody detects phosphorylation in response to high- and low-efficacy agonists and after PKC activation. S332/S334 phosphorylation is a key regulator of C5a1 desensitization, β-arrestin recruitment and internalization.

  Alternative Names C5a1, C5AR1, Complement C5a Receptor 1, C5a Anaphylatoxin... more

 

Alternative Names C5a1, C5AR1, Complement C5a Receptor 1, C5a Anaphylatoxin Chemotactic Receptor
IUPHAR Target ID  32
UniProt ID  P21730
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human C5a1 around the phosphorylation site of Ser332/Ser334
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine332/Serine334 phosphorylation of the Complement C5a Receptor... more

Figure 1. Agonist-induced Serine332/Serine334 phosphorylation of the Complement C5a Receptor 1. Upper panel, HEK293 cells stably expressing the Complement C5a Receptor 1 (C5a1) were either not exposed or exposed to 1 µM specific Complement C5a receptor agonist CO28 or 10 μM Forskolin or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pS332/pS334-C5a1 antibody (7TM0032B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-C5a1 antibody (7TM0032N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Complement C5a Receptor 1 phosphorylation using two phosphosite-specific antibodies. Upper two panels, HEK293 cells stably expressing the Complement C5a Receptor 1 (C5a1) were either not exposed or exposed to increasing concentrations of complement fragment C5a ranging from 1 nM to 100 nM for 30 minutes. Cells were lysed and immunoblotted with the anti-pT324/pS327-C5a1 antibody (7TM0032A) or anti-pS332/pS334-C5a1 antibody (7TM0032B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-C5a1 antibody (7TM0032N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 3. Analysis of agonist-dependent Complement C5a Receptor 1 phosphorylation using two phosphosite-specific antibodies. Upper two panels, HEK293 cells stably expressing the Complement C5a Receptor 1 (C5a1) were either not exposed or exposed to 10 µM CO28 or 100 nM complement fragment C5a or 100 nM des-Arg C5a (C5a lacking the c-terminal arginine) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT324/pS327-C5a1 antibody (7TM0032A) or anti-pS332/pS334-C5a1 antibody (7TM0032B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-C5a1 antibody (7TM0032N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Be the first to decipher the C5a1 phosphorylation barcode and let us know. more

Be the first to decipher the C5a1 phosphorylation barcode and let us know.

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