C5a2 (non-phospho), Complement C5a Receptor 2 Antibody

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Preview: Validation of the Complement C5a Receptor 2 in transfected HEK293 cells.

Preview: Immunocytochemical identification of the Complement C5a Receptor 2 in transfected HEK293 cells.

Validation of the Complement C5a Receptor 2 in transfected HEK293 cells.

Immunocytochemical identification of the Complement C5a Receptor 2 in transfected HEK293 cells.

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Order number: 7TM0033N
Content: 100 µl
Host: Rabbit

The non-phospho-C5a2 receptor antibody is directed against the distal part of the... more

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The non-phospho-C5a2 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human C5a2. It can be used to detect total C5a2 receptors in Western blots independent of phosphorylation. The non-phospho-C5a2 antibody can also be used to isolate and enrich C5a2 receptors from cell and tissue lysates.

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Alternative Names C5a2, C5AR2, Complement C5a Receptor 2, C5a Anaphylatoxin... more

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Alternative Names	C5a2, C5AR2, Complement C5a Receptor 2, C5a Anaphylatoxin Chemotactic Receptor 2
IUPHAR Target ID	33
UniProt ID	Q9P296
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents carboxyl-terminal tail of human C5a2
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Complement C5a Receptor 2 in transfected HEK293 cells. Native... more

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Figure 1. Validation of the Complement C5a Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Complement C5a Receptor 2 (C5a2) were lysed and immunoblotted with the phosphorylation-independent anti-C5a2 antibody (7TM0033N-WB) at a dilution of 1:1000.

Figure 2. Immunocytochemical identification of the Complement C5a Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Complement C5a Receptor 2 (C5a2) were immunocytochemically stained with the phosphorylation-independent anti-C5a2 antibody (7TM0033N-WB) at a dilution of 1:200.

Nürge B, Schulz AL, Kaemmerer D, Sänger J, Evert K, Schulz S, Lupp A. Immunohistochemical... more

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Nürge B, Schulz AL, Kaemmerer D, Sänger J, Evert K, Schulz S, Lupp A. Immunohistochemical identification of complement peptide C5a receptor 1 (C5aR1) in non-neoplastic and neoplastic human tissues. PLoS One. 2021 Feb 19;16(2):e0246939. doi: 10.1371/journal.pone.0246939. PMID: 33606748; PMCID: PMC7894821.

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