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pT335-FPR2 (phospho-Formylpeptide Receptor 2 Antibody)

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Agonist-induced Threonine335 phosphorylation of FPR2 Receptor
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  • 7TM0223A
  • 100 µl
  • Rabbit
Threonine335 (T335) is a major phosphorylation site of the FPR2 receptor. The pT335-FPR2... more

Threonine335 (T335) is a major phosphorylation site of the FPR2 receptor. The pT335-FPR2 antibody detects phosphorylation in response to high-efficacy agonists. T335 phosphorylation is a key regulator of FPR2 desensitization, β-arrestin recruitment and internalization.

  Alternative Names ALXR, FPRL1, FPRH2, Formyl-peptide Receptor-like 1,... more

 

Alternative Names ALXR, FPRL1, FPRH2, Formyl-peptide Receptor-like 1, Formyl-peptide Receptor 2
IUPHAR Target ID  223
UniProt ID  P25090
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  -
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human FPR2 around the phosphorylation site Thr335.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine335 phosphorylation of FPR2 Receptor. Upper panel, HEK293... more

Figure 1. Agonist-induced Threonine335 phosphorylation of FPR2 Receptor. Upper panel, HEK293 cells stably expressing the FPR2-Receptor (FPR2) were either not exposed or exposed to 1 µM of specific FPR-Receptor agonist fMet-Leu-Phe for 30 minutes. Cells were lysed and immunoblotted with the anti-pT335-FPR2 antibody (7TM0223A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-FPR2 antibody to confirm equal loading of the gel.

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