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pS305/pS306-FFA3 (phospho-FFA3 Antibody)

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Agonist-induced Serine305/Serine306 phosphorylation of the FFA Receptor 3
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  • 7TM0227A
  • 100 µl
  • Rabbit
Serine305/Serine306 (S305/S306) is major phosphorylation site of the FFA Receptor 3 (FFA3). The... more

Serine305/Serine306 (S305/S306) is major phosphorylation site of the FFA Receptor 3 (FFA3). The pS305/pS306-FFA3 antibody detects phosphorylation in response to agonists. S305/S306 phosphorylation is likely to be involved in efficient ligand sequestration by FFA3.

  Alternative Names FFAR3, Free Fatty Acid Receptor 3, GPCR41 IUPHAR... more

 

Alternative Names FFAR3, Free Fatty Acid Receptor 3, GPCR41
IUPHAR Target ID  227
UniProt ID  O14843
Western Blot (WB)  1:500
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human FFA3 around the phosphorylation site of Ser305/Ser306.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine305/Serine306 phosphorylation of the FFA Receptor 3. Upper... more

Figure 1. Agonist-induced Serine305/Serine306 phosphorylation of the FFA Receptor 3. Upper panel, Flp-In TREx 293 harbouring human FFA3-eYFP induced to express the receptor construct (+ dox) were treated with vehicle, 2mM propanoic acid (C3) or 5µM TUG-1907 for 5 min. Cells were lysed and receptor construct enriched with a GFP-trap, before immunoblotting with the anti-pS305/pS306-FFA3 antibody (7TM0227A) at a dilution of 1:500. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-GFP antibody to confirm equal loading of the gel. TUG-1097 is described in J. Med Chem 63(7):3577-3595

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