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pT328/pS329-FFA3 (phospho-FFA3 Antibody)

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 Agonist-induced Threonine328/Serine329 phosphorylation of the FFA Receptor 3
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  • 7TM0227B
  • 100 µl
  • Rabbit
Threonine328/Serine329 (T328/S329) is major phosphorylation site of the FFA Receptor 3 (FFA3).... more

Threonine328/Serine329 (T328/S329) is major phosphorylation site of the FFA Receptor 3 (FFA3). The pT328/pS329-FFA3 antibody detects phosphorylation in response to agonists. T328/S329 phosphorylation is likely to be involved in efficient ligand sequestration by FFA3.

  Alternative Names FFAR3, Free Fatty Acid Receptor 3, GPCR41 IUPHAR... more

 

Alternative Names FFAR3, Free Fatty Acid Receptor 3, GPCR41
IUPHAR Target ID  227
UniProt ID  O14843
Western Blot (WB)  1:500
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human FFA3 around the phosphorylation site of Thr328/Ser329.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine328/Serine329 phosphorylation of the FFA Receptor 3. Upper... more

Figure 1. Agonist-induced Threonine328/Serine329 phosphorylation of the FFA Receptor 3. Upper panel, Flp-In TREx 293 harbouring human FFA2-eYFP induced to express the receptor construct (+ dox) were treated with vehicle, 2mM propanoic acid (C3) or 5µM TUG-1907 for 5 min. Cells were lysed and receptor construct enriched with a GFP-trap, before immunoblotting with the anti-pT328/pS329-FFA3 antibody (7TM0227B) at a dilution of 1:500. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-FFA3 antibody to confirm equal loading of the gel. TUG-1097 is described in J. Med Chem 63(7):3577-3595

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