Premium Phosphosite-Specific 7TM Antibodies
Novel Tools for Your GPCR Research
Select Your Country of Delivery below

pS356/pT357-KOP (phospho-κ-Opioid Receptor Antibody)

Citations
$ 375.00 *

Prices plus VAT plus shipping costs

Ready to ship today,
Delivery time appr. 5-8 days

  • 7TM0318A
  • 100 µl
  • Rabbit
Serine356/Threonine357 is a major phosphorylation site of the kappa-opioid receptor (KOP). The... more

Serine356/Threonine357 is a major phosphorylation site of the kappa-opioid receptor (KOP). The pS356/pT357-KOP antibody detects phosphorylation in response to agonists as well as after PKC activation. S356/T357 phosphorylation is a key regulator of KOP desensitization, β-arrestin recruitment and internalization.

  Alternative Names KOP, OPRK1, κ-Opioid Receptor IUPHAR Target ID... more

 

Alternative Names KOP, OPRK1, κ-Opioid Receptor
IUPHAR Target ID  318
UniProt ID  P41145 (human) P33534 (mouse) P34975 (rat)
Western Blot (WB)  1:1000
Species Reactivity   Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human KOP around the phosphorylation site of Ser356/Thr357
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine356/Threonine357 phosphorylation of the κ-Opioid Receptor.... more

Figure 1. Agonist-induced Serine356/Threonine357 phosphorylation of the κ-Opioid Receptor. Upper panel, HEK293 cells stably expressing the κ-Opioid Receptor (KOP) were either not exposed or exposed to 10 μM of selective κ-opioid receptor agonist U50,488H for 30 minutes. Cells were lysed and immunoblotted with the anti-pS356/pT357-KOP antibody (7TM0318A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-KOP antibody (7TM0318N-WB) to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent κ-Opioid Receptor phosphorylation using two phosphosite-specific antibodies. Upper two panels, HEK293 cells stably expressing the κ-Opioid Receptor (KOP) were either not exposed or exposed to increasing concentrations of selective κ-opioid receptor agonist U50,488H ranging from 0.1 nM to 1 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS356/pT357-KOP antibody (7TM0318A) or anti-pT363-KOP antibody (7TM0318B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-KOP antibody (7TM0318N-WB) to confirm equal loading of the gel.

Read, write and discuss reviews... more
Customer evaluation for "pS356/pT357-KOP (phospho-κ-Opioid Receptor Antibody)"
Write an evaluation
Evaluations will be activated after verification.

The fields marked with * are required.

Recently viewed