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pS346-NOP (phospho-Nociceptin/Orphanin FQ Receptor Antibody)

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  • 7TM0320A
  • 100 µl
  • Rabbit
Serine346 (S346) is a major phosphorylation site of the nociceptin/orphanin FQ peptide receptor... more

Serine346 (S346) is a major phosphorylation site of the nociceptin/orphanin FQ peptide receptor (NFQ receptor, NOP). The pS346-NOP antibody detects phosphorylation in response to agonists as well as after PKC activation. S346 phosphorylation is a key regulator of NOP desensitization, β-arrestin recruitment and internalization.

  Alternative Names NOP, OPRL1, Nociceptin/Orphanin FQ Receptor IUPHAR... more

 

Alternative Names NOP, OPRL1, Nociceptin/Orphanin FQ Receptor
IUPHAR Target ID  320
UniProt ID  P41146 (human) P35377 (mouse) P35370 (rat)
Western Blot (WB)  1:1000
Species Reactivity   Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human NOP around the phosphorylation site of Ser346
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine346 phosphorylation of the Nociceptin/Orphanin FQ Receptor.... more

Figure 1. Agonist-induced Serine346 phosphorylation of the Nociceptin/Orphanin FQ Receptor. Upper panel, HEK293 cells stably expressing the Nociceptin/Orphanin FQ Receptor (NOP) were either not exposed or exposed to 10 μM Nociceptin/Orphanin FQ or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pS346-NOP antibody (7TM0320A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-NOP antibody (7TM0320N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Nociceptin/Orphanin FQ Receptor phosphorylation using a panel of phosphosite-specific antibodies. Upper three panels, HEK293 cells stably expressing the Nociceptin/Orphanin FQ Receptor (NOP) were either not exposed or exposed to increasing concentrations of Nociceptin/Orphanin FQ ranging from 1 nM to 10 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS346-NOP antibody (7TM0320A) or anti-pS351-NOP antibody (7TM0320B) or anti-pT362/pS363-NOP antibody (7TM0320C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-NOP antibody (7TM0320N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

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