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pS298/pS301-mGPR35 (phospho-mouse GPR35 Antibody)

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  • 7TM0102B
  • 100 µl
  • Rabbit
Serine298/Serine301 (S298/S301) is a major phosphorylation site of the mouse GPR35 receptor.... more

Serine298/Serine301 (S298/S301) is a major phosphorylation site of the mouse GPR35 receptor. The pS298/pS301-mGPR35 antibody detects phosphorylation in response to high-efficacy agonists. S298/S301 phosphorylation is a key regulator of mouse GPR35 desensitization, β-arrestin recruitment and internalization.

  Alternative Names GPR35, G-protein coupled Receptor 35 IUPHAR Target... more

 

Alternative Names GPR35, G-protein coupled Receptor 35
IUPHAR Target ID  102
UniProt ID  Q9ES90
Western Blot (WB)  1:1000
Immunocytochemistry (ICC) 1:200
Species Reactivity   Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from mouse GPR35 around the phosphorylation site Ser298/Ser301.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine298/Serine301 phosphorylation of mouse GPR35 Receptor. Upper... more

Figure 1. Agonist-induced Serine298/Serine301 phosphorylation of mouse GPR35 Receptor. Upper panel, HEK293 cells transiently expressing the eYFP-tagged mouse GPR35-Receptor were either not exposed (vehicle) or exposed to 100 nM of specific GPR35-Receptor agonist zaprinast for 5 minutes. Cells were lysed and receptor constuct enriched with a GFP-trap before immunoblotting with the anti-pS298/pS301-mGPR35 antibody (7TM0102B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-GFP antibody to confirm equal loading of the gel.

Figure 2. Immunocytochemical Identification of Serine298/Serine301 phosphorylation of mouse GPR35 Receptor. Flp-ln TREx 293 cells stably expressing hGPR35a-HA were either treated with vehicle or 100 μM zaprinast for 5 min and immunocytochemically stained with anti-pS298/pS301-mGPR35 antibody (7TM0102A) at a dilution of 1:200.

Divorty N, Jenkins L, Ganguly A, Butcher AJ, Hudson BD, Schulz S, Tobin AB, Nicklin SA,... more

Divorty N, Jenkins L, Ganguly A, Butcher AJ, Hudson BD, Schulz S, Tobin AB, Nicklin SA, Milligan G. Agonist-induced phosphorylation of orthologues of the orphan receptor GPR35 functions as an activation sensor. J Biol Chem. 2022 Mar;298(3):101655. doi: 10.1016/j.jbc.2022.101655. Epub 2022 Jan 29. PMID: 35101446; PMCID: PMC8892012.

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