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pT372/pS373-EP3 (phospho-EP3 Prostanoid Receptor Antibody)

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Agonist-induced Threonine372/Serine373 phosphorylation of the EP3 Prostanoid Receptor
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  • 7TM0342B
  • 100 µl
  • Rabbit
Threonine372/Serine373 (T372/S373) is major phosphorylation site of the EP3 Prostanoid Receptor... more

Threonine372/Serine373 (T372/S373) is major phosphorylation site of the EP3 Prostanoid Receptor (EP3). The pT372/pS373-EP3 antibody detects phosphorylation in response to agonists. T372/S373 phosphorylation is likely to be involved in efficient ligand sequestration by EP3.

  Alternative Names PGE Receptor EP3 subtype, Prosaglandin E Receptor 3... more

 

Alternative Names PGE Receptor EP3 subtype, Prosaglandin E Receptor 3
IUPHAR Target ID  342
UniProt ID  P43115
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human EP around the phosphorylation site of Thr372/Ser373.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threon ine372/Serine373 phosphorylation of the EP3 Prostanoid... more

Figure 1. Agonist-induced Threonine372/Serine373 phosphorylation of the EP3 Prostanoid Receptor. Upper panel, HEK293 cells stably expressing the EP3 Prostanoid receptor (EP3) were either not exposed or exposed to 10 µM Misoprostol for 30 minutes. Cells were lysed and immunoblotted with the anti-pT372/pS373-EP3 antibody (7TM0342B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-EP3 antibody to confirm equal loading of the gel.

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