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pS418-PAR1 (phospho-Proteinase-Activated Receptor 1 Antibody)

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Agonist-induced Serine418 phosphorylation of the Proteinase-Activated Receptor 1
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  • 7TM0347B
  • 100 µl
  • Rabbit
Serine418 (S418) is major phosphorylation site of the Proteinase-Activated Receptor 1... more

Serine418 (S418) is major phosphorylation site of the Proteinase-Activated Receptor 1 (PAR1). The pS418-PAR1 antibody detects phosphorylation in response to agonists. S418 phosphorylation is likely to be involved in efficient ligand sequestration by PAR1.

  Alternative Names Coagulation Factor II Receptor, Protease-Activated... more

 

Alternative Names Coagulation Factor II Receptor, Protease-Activated Receptor 1
IUPHAR Target ID  347
UniProt ID  P25116
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human PAR1 around the phosphorylation site of Ser418.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine418 phosphorylation of the Proteinase-Activated Receptor 1.... more

Figure 1. Agonist-induced Serine418 phosphorylation of the Proteinase-Activated Receptor 1. Upper panel, HEK293 cells stably expressing the Proteinase-activated receptor 1 (PAR1) were either not exposed or exposed to 100 nM TFLLRNH2 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS418-PAR1 antibody (7TM0347B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-PAR1 antibody (7TM0347N) to confirm equal loading of the gel.

Be the first to analyze the PAR1 phosphorylation using our anti-pS418-PAR1... more

Be the first to analyze the PAR1 phosphorylation using our anti-pS418-PAR1 antibody and let us know.

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