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pT375/pS376-PAR2 (phospho-Proteinase-Activated Receptor 2 Antibody)

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Agonist-induced Threonine375/Serine376 phosphorylation of the Proteinase-Activated Receptor 2
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  • 7TM0348A
  • 100 µl
  • Rabbit
Threonine375/Serine376 (T375/S376) is major phosphorylation site of... more

Threonine375/Serine376 (T375/S376) is major phosphorylation site of the Proteinase-Activated Receptor 2 (PAR2). The pT375/pS376-PAR2 antibody detects phosphorylation in response to agonists. T375/S376 phosphorylation is likely to be involved in efficient ligand sequestration by PAR2.

  Alternative Names Coagulation Factor II Receptor-like 1, Protease-Activated... more

 

Alternative Names Coagulation Factor II Receptor-like 1, Protease-Activated Receptor 2
IUPHAR Target ID  348
UniProt ID  P55085
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human PAR2 around the phosphorylation site of Thr375/Ser376.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine375/Serine376 phosphorylation of the Proteinase-Activated... more

Figure 1. Agonist-induced Threonine375/Serine376 phosphorylation of the Proteinase-Activated Receptor 2. Upper panel, HEK293 cells stably expressing the Proteinase-activated receptor 2 (PAR2) were either not exposed or exposed to 100 nM AC264613 for 30 minutes. Cells were lysed and immunoblotted with the anti-pT375/pS376-PAR2 antibody (7TM0348A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-PAR2 antibody to confirm equal loading of the gel.

Be the first to analyze the ACKR2 phosphorylation using our anti-pS348/pS350-ACKR2... more

Be the first to analyze the ACKR2 phosphorylation using our anti-pS348/pS350-ACKR2 antibody and let us know.

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