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pT348-SST3 (phospho-Somatostatin Receptor 3 Antibody)

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  • 7TM0357B
  • 100 µl
  • Rabbit
Threonine348 (T348) is a major phosphorylation site of the somatostatin receptor 3 (SST3). The... more

Threonine348 (T348) is a major phosphorylation site of the somatostatin receptor 3 (SST3). The pT348-SST3 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. T348 phosphorylation is a key regulator of SST3 desensitization, β-arrestin recruitment and internalization.

  Alternative Names SST3, SSTR3, Somatostatin Receptor 3 IUPHAR... more

 

Alternative Names SST3, SSTR3, Somatostatin Receptor 3
IUPHAR Target ID  357
UniProt ID  P32745
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human SST3 around the phosphorylation site of Thr348
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine348 phosphorylation of the Somatostatin Receptor 3. Upper... more

Figure 1. Agonist-induced Threonine348 phosphorylation of the Somatostatin Receptor 3. Upper panel, HEK293 cells stably expressing the Somatostatin Receptor 3 (SST3) were either not exposed or exposed to 1 μM SRIF (somatotropin-release inhibiting factor) or 1 μM of artificial somatostatin receptor agonist Octreotide for 30 minutes. Cells were lysed and immunoblotted with the anti-pT348-SST3 antibody (7TM0357B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-SST3 antibody (7TM0357N-WB) at a dilution of 1:1000 to confirm equal loading of the gel. Note, SST3 receptor forms stable dimers.

Figure 2. Analysis of dose-dependent Somatostatin Receptor 3 phosphorylation. Upper panel, HEK293 cells stably expressing the Somatostatin Receptor 3 (SST3) were either not exposed or exposed to increasing concentrations of SRIF (somatotropin-release inhibiting factor) ranging from 1 nM to 1 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pT348-SST3 antibody (7TM0357B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-SST3 antibody (7TM0357N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

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