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pS398/pS399-NK3 (phospho-Tachykinin Receptor 3 Antibody)

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Agonist-induced Serine398/Serine399 phosphorylation of the NK 3 Recept
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  • 7TM0362B
  • 100 µl
  • Rabbit
Serine398/Serine399 (S398/S399) is major phosphorylation site of the Tachykinin Receptor 3... more

Serine398/Serine399 (S398/S399) is major phosphorylation site of the Tachykinin Receptor 3 (NK3). The pS398/pS399-NK3 antibody detects phosphorylation in response to agonists. S398/S399 phosphorylation is likely to be involved in efficient ligand sequestration by NK3.

  Alternative Names NMKR, TAC3R, Neurokinin B Receptor, Neuromedin K Receptor... more

 

Alternative Names NMKR, TAC3R, Neurokinin B Receptor, Neuromedin K Receptor
IUPHAR Target ID  362
UniProt ID  P29371
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human NK3 around the phosphorylation site of Ser398/Ser399.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine398/Serine399 phosphorylation of the NK 3 Receptor. Upper... more

Figure 1. Agonist-induced Serine398/Serine399 phosphorylation of the NK 3 Receptor. Upper panel, HEK293 cells stably expressing the NK3-Receptor (NK3) were either not exposed or exposed to 1 µM of specific NK3-Receptor agonist Neurokinin B for 30 minutes. Cells were lysed and immunoblotted with the anti-pS398/pS399-NK3 antibody (7TM0362B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-NK3 antibody to confirm equal loading of the gel.

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