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pS450/pS452-PAC1 (phospho-PACAP Receptor 1 Antibody)

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Agonist-induced Serine450/Serine452 phosphorylation of the PACAP Receptor 1
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  • 7TM0370C
  • 100 µl
  • Rabbit
Serine450/Serine452 (S450/S452) is major phosphorylation site of the PACAP Receptor 1 (PAC1).... more

Serine450/Serine452 (S450/S452) is major phosphorylation site of the PACAP Receptor 1 (PAC1). The pS450/pS452-PAC1 antibody detects phosphorylation in response to agonists. S450/S452 phosphorylation is likely to be involved in efficient ligand sequestration by PAC1.

  Alternative Names PAC1, PVR1, PACAP Receptor 1, Pituitary adenylate cyclase... more

 

Alternative Names PAC1, PVR1, PACAP Receptor 1, Pituitary adenylate cyclase activating polypeptide 1 receptor
IUPHAR Target ID  370
UniProt ID  P41586
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human PAC1 around the phosphorylation site of Ser450/Ser452.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine450/Serine452 phosphorylation of the PACAP Receptor 1. Upper... more

Figure 1. Agonist-induced Serine450/Serine452 phosphorylation of the PACAP Receptor 1. Upper panel, HEK293 cells stably expressing the PACAP  receptor 1  (PAC1) were either not exposed or exposed to 100 nM PACAP for 30 minutes. Cells were lysed and immunoblotted with the anti-pS450/pS452-PAC1 antibody (7TM0370C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-PAC1 antibody (7TM0370N) to confirm equal loading of the gel.

Be the first to analyze the ACKR2 phosphorylation using our anti-pS348/pS350-ACKR2... more

Be the first to analyze the ACKR2 phosphorylation using our anti-pS348/pS350-ACKR2 antibody and let us know.

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