VPAC2 (non-phospho), VIP Receptor 2 Antibody

KO-Validated

Preview: Validation of the VIP Receptor 2 in transfected HEK293 cells

Preview: Immunocytochemical identification of VIP Receptor 2 in HEK293 cells

Preview: Immunohistochemical identification of VIP Receptor 2 in small intestine

Preview: Immunohistochemical identification of VIP Receptor 2 in breast carcinoma

Validation of the VIP Receptor 2 in transfected HEK293 cells

Immunocytochemical identification of VIP Receptor 2 in HEK293 cells

Immunohistochemical identification of VIP Receptor 2 in small intestine

Immunohistochemical identification of VIP Receptor 2 in breast carcinoma

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Order number: 7TM0372N
Content: 100 µl
Host: Rabbit

The VPAC2 antibody is directed against the distal end of the carboxyl-terminal tail of mouse,... more

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The VPAC2 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human VIP receptor 2. It can be used to detect total VPAC2 receptors in Western blots independent of phosphorylation. The VPAC2 antibody can also be used to isolate and enrich VPAC2 receptors from cell and tissue lysates. It also detects VPAC2 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names VPAC2, VIP Receptor 2, Vasoactive Intestinal Peptide... more

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Alternative Names	VPAC2, VIP Receptor 2, Vasoactive Intestinal Peptide Receptor 2
IUPHAR Target ID	372
UniProt ID	P41587 (human) P41588 (mouse) P35000 (rat)
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide with sequence LQFHRGSRAQSFLQTETSVI corresponds to amino acids 419-438 of VPAC2.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the VIP Receptor 2 in transfected HEK293 cells. Native HEK293 cells... more

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Figure 1. Validation of the VIP Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the VIP Receptor 2 (VPAC2) were lysed and immunoblotted with the phosphorylation-independent anti-VPAC2 antibody (7TM0372N) at a dilution of 1:1000.

Figure 2. Immunocytochemical identification of VIP Receptor 2 in HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the VIP receptor 2 (VPAC2) were immunocytochemically stained with anti-VPAC2 (VIP receptor 2) antibody (7TM0372N) at a dilution of 1:200. Note, VPAC2 receptors were confined to the plasma membrane in transfected cells.

Figure 3. Immunohistochemical identification of VIP Receptor 2 in small intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-VPAC2 (VIP receptor 2) antibody (7TM0372N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, VPAC2 receptors were detected at the plasma membrane of neuroendocrine cells in small intestine.

Figure 4. Immunohistochemical identification of VIP Receptor 2 in breast carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-VPAC2 (VIP receptor 2) antibody (7TM0372N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, VPAC2 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.