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Delivery time appr. 5-8 days
- Order number: 7TM00CLX
If you need stable cell lines for your GPCR target, please contact us. 7TM can provide stably transfected HEK293 cells for many GPCR targets. Please inquire for availablity.
7TM stable cell lines exhibit > 90% positive cells and are negatively tested for mycoplasma. 7TM stable cell lines are shipped on dry ice.
Figure 1. Immuncytochemical verification of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged metabotropic Glutamate Receptor 2 (HA-mGLU2), HA-tagged Calcitonin Receptor-like Receptor (HA-CALCRL), HA-tagged Corticotropin-releasing Factor Receptor 1 (HA-CRF1), HA-tagged orphan G Protein-coupled Receptor 20 (HA-GPR20), metabotropic Glutamate Receptor 4 (HA-mGLU4) or HA-tagged Dopamine receptor 1 (HA-D1) were immunocytochemically stained with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:200. Note, the receptors are located at the plasma membrane of the cells.
Figure 2. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged C-C chemokine receptor 5 (HA-CCR5), HA-tagged C-C chemokine receptor 7 (HA-CCR7), HA-tagged C-C chemokine receptor 8 (HA-CCR8), HA-tagged C-C chemokine receptor 9 (HA-CCR9), HA-tagged C-C chemokine receptor 10 (HA-CCR10), HA-tagged muscarinic acetylcholine receptor 3 (HA-M3), HA-tagged histamine H1 receptor (HA-H1), HA-tagged C-X3-C motif chemokine receptor 1 (HA-CX3CR1) or HA-tagged hypocretin receptor 1/orexin receptor 1 (HA-OX1) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.
Figure 3. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged Adenosin Diphosphate Receptor 4 (HA-P2Y4), HA-tagged Adenosin Diphosphate Receptor 12 (HA-P2Y12), HA-tagged Alpha-1 Adrenergic Receptor A (HA-α1A), HA-tagged Dopamine Receptor 5 (HA-D5) or HA-tagged Histamine H2 receptor (HA-H2) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.
Figure 4. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged Hypocretin Receptor 1/Orexin Receptor 1 (HA-OX1), HA-tagged Canabinoid Receptor 1 (HA-CB1), HA-tagged Canabinoid Receptor 1 (HA-CB2), HA-tagged Galanin Receptor 1 (HA-GAL1) or HA-tagged G Protein-coupled Estrogen Receptor (HA-GPER) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.
Figure 5. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged Serotonin Receptor 4 (HA-5-HT4), HA-tagged Serotonin Receptor 6 (HA-5-HT6), HA-tagged Serotonin Receptor 2A (HA-5-HT2A), HA-tagged Cholecystokinin Receptor 1 (HA-CCK1) or HA-tagged Cholecystokinin Receptor 2 (HA-CCK2) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.
7TM Stable Cell Lines are available for all our 7TM Antibodies. Please inquire for all other GPCRs. Delivery time for stable cell lines is 10 to 14 days.