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pS364/pS366-EP4 (phospho-EP4 Prostanoid Receptor Antibody)

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Agonist-induce Serine364/Serine366 phosphorylation of the EP4 Prostanoid Receptor
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  • 7TM0343C
  • 100 µl
  • Rabbit
Serine364/Serine366 (S364/S366) is major phosphorylation site of the EP4 Prostanoid Receptor... more

Serine364/Serine366 (S364/S366) is major phosphorylation site of the EP4 Prostanoid Receptor (EP4). The pS364/pS366-EP4 antibody detects phosphorylation in response to agonists. S364/S366 phosphorylation is likely to be involved in efficient ligand sequestration by EP4.

  Alternative Names PGE Receptor EP4 subtype, Prostanoid EP4 Receptor... more

 

Alternative Names PGE Receptor EP4 subtype, Prostanoid EP4 Receptor
IUPHAR Target ID  343
UniProt ID  P35408
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human EP around the phosphorylation site of Ser364/Ser366.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induce Serine364/ Serine366  phosphorylation of the EP4 Prostanoid... more

Figure 1. Agonist-induce Serine364/Serine366 phosphorylation of the EP4 Prostanoid Receptor. Upper panel, HEK293 cells stably expressing the EP4 Prostanoid receptor (EP4) were either not exposed or exposed to 10 µM Misoprostol for 30 minutes. Cells were lysed and immunoblotted with the anti-pS364/pS366-EP4 antibody (7TM0343C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-EP4 antibody to confirm equal loading of the gel.

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Be the first to analyze the EP4 phosphorylation using our anti-pS364/pS366-EP4 antibody and let us know.

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