CRF1 (IHC-grade), Corticotropin-Releasing Factor Receptor 1 Antibody

Figure 1. Immunohistochemical identification of Corticotropin-Releasing Factor Receptor 1 in anterior pituitary. Sections were dewaxed, microwaved in citric acid, and incubated with anti-CRF1 (Corticotropin-Releasing Factor Receptor 1) antibody (7TM0212N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, CRF1 receptors were detected at the plasma membrane of a subpopulation of anterior pituitary cells.

Figure 2. Validation of the Corticotropin-Releasing Factor Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Corticotropin-Releasing Factor Receptor 1 (CRF1) were lysed and immunoblotted with the phosphorylation-independent anti-CRF1 antibody (7TM0212N-IC) at a dilution of 1:1000.

Figure 3. Immunocytochemical identification of Corticotropin-Releasing Factor Receptor 1 in HEK293 cells. HEK293 cells stably expressing the Corticotropin-Releasing Factor Receptor 1 (CRF1) were either not exposed or exposed to 100 nM CRF for 30 min and immunocytochemically stained with anti-CRF1 antibody (7TM0212N-IC) at a dilution of 1:200. Note, CRF1 receptors were confined to the plasma membrane in untreated cells (0 min).CRF1 receptors were seen in perinuclear clusters of vesicles after 30 min CRF exposure.