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pS363/pT364-BB2 (phospho-Bombesin Receptor 2 Antibody)

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  • 7TM0039C
  • 100 µl
  • Rabbit
Serine363/Threonine364 (S363/T364) is major phosphorylation site of the Bombesin Receptor 2... more

Serine363/Threonine364 (S363/T364) is major phosphorylation site of the Bombesin Receptor 2 (BB2). The pS363/pT364-BB2 antibody detects phosphorylation in response to agonists. S363/T364 phosphorylation is likely to be involved in efficient ligand sequestration by BB2.

  Alternative Names BB2, GRPR, Bombesin Receptor 2, Gastrin-releasing Peptide... more

 

Alternative Names BB2, GRPR, Bombesin Receptor 2, Gastrin-releasing Peptide Receptor
IUPHAR Target ID  39
UniProt ID  P30550
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human BB2 around the phosphorylation site of Ser363/Thr364.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine363/Threonine364 phosphorylation of the Bombesin Receptor 2.... more

Figure 1. Agonist-induced Serine363/Threonine364 phosphorylation of the Bombesin Receptor 2. Upper panel, HEK293 cells stably expressing the Bombesin receptor 2 (BB2) were either not exposed or exposed to 100 nM Bombesin for 30 minutes. Cells were lysed and immunoblotted with the anti-pS363/pT364-BB2 antibody (7TM0039B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-BB2 antibody (7TM0039N) to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Bombesin Receptor 2 phosphorylation using two phosphosite-specific antibodies. Upper two panels, HEK293 cells stably expressing the Bombesin Receptor 2 (BB2) were either not exposed or exposed to increasing concentrations of Bombesin ranging from 1 nM to 10 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pT359/pS360-BB2 antibody (7TM0039A) or anti-pS363/pS364-BB2 antibody (7TM0039B)  at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-BB2 antibody (7TM0039N) at a dilution of 1:1000 to confirm equal loading of the gel.

Be the first to analyze the ACKR2 phosphorylation using our anti-pS348/pS350-ACKR2... more

Be the first to analyze the ACKR2 phosphorylation using our anti-pS348/pS350-ACKR2 antibody and let us know.

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