Serine363/Threonine364 (S363/T364) is major phosphorylation site of the Bombesin Receptor 2 (BB2). The pS363/pT364-BB2 antibody detects phosphorylation in response to agonists. S363/T364 phosphorylation is likely to be involved in efficient ligand sequestration by BB2.
|Alternative Names||BB2, GRPR, Bombesin Receptor 2, Gastrin-releasing Peptide Receptor|
|IUPHAR Target ID||39|
|Western Blot (WB)||1:1000|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide derived from human BB2 around the phosphorylation site of Ser363/Thr364.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide|
|Storage conditions||short-term 4°C, long-term -20°C|
Figure 1. Agonist-induced Serine363/Threonine364 phosphorylation of the Bombesin Receptor 2. Upper panel, HEK293 cells stably expressing the Bombesin receptor 2 (BB2) were either not exposed or exposed to 100 nM Bombesin for 30 minutes. Cells were lysed and immunoblotted with the anti-pS363/pT364-BB2 antibody (7TM0039B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-BB2 antibody (7TM0039N) to confirm equal loading of the gel.
Figure 2. Analysis of dose-dependent Bombesin Receptor 2 phosphorylation using two phosphosite-specific antibodies. Upper two panels, HEK293 cells stably expressing the Bombesin Receptor 2 (BB2) were either not exposed or exposed to increasing concentrations of Bombesin ranging from 1 nM to 10 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pT359/pS360-BB2 antibody (7TM0039A) or anti-pS363/pS364-BB2 antibody (7TM0039B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-BB2 antibody (7TM0039N) at a dilution of 1:1000 to confirm equal loading of the gel.
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