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Epitope-Tag Antibodies

For many GPCRs specific antibodies do not exist. For these receptors epitope-tag antibodies are versatile tools to detect total receptor protein in Western blots independent of phosphorylation. Epitope-tag antibodies can also be used to isolate and enrich receptors from cell and tissue lysates. Epitope-tag antibodies can also be used to localize tagged receptors in cultured cells and tissue sections by immunohistochemistry. These antibodies can also be used for surface labeling of N-terminally tagged receptors to quantify receptor trafficking using ELISA or flow cytometry.

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Validation of FLAG-tagged µ-Opioid Receptor in transfected HEK293 cells.
FLAG-Tag, Anti-FLAG Epitope Tag Antibody
The anti-FLAG tag antibody is directed against the sequence DYKDDDDK. The anti-FLAG antibody can be used to detect total receptors in Western blots independent of phosphorylation. The anti-FLAG tag antibody can also be used to isolate...
$ 150.00 *
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Citations
Validation of HA-tagged µ-Opioid Receptor in stable HEK293 cell lines.
HA-Tag, Anti-HA Epitope Tag Antibody
The anti-HA tag antibody is directed against amino acids 98-106 of the human influenza hemagglutinin (HA). The HA tag can used as a general epitope tag in expression vectors. The anti-HA antibody can be used to detect total receptors in...
$ 150.00 *
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Selected References

Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOPreceptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists. Sci Signal. 2019 Mar 26;12(574). pii: eaau8072. doi: 10.1126/scisignal.aau8072. PubMed PMID: 30914485; PubMed Central PMCID: PMC6934085.

Miess E, Gondin AB, Yousuf A, Steinborn R, Mösslein N, Yang Y, Göldner M, Ruland JG, Bünemann M, Krasel C, Christie MJ, Halls ML, Schulz S, Canals M. Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization. Sci Signal. 2018 Jul 17;11(539). pii: eaas9609. doi: 10.1126/scisignal.aas9609. PubMed PMID: 30018083.

Just S, Illing S, Trester-Zedlitz M, Lau EK, Kotowski SJ, Miess E, Mann A, Doll C, Trinidad JC, Burlingame AL, von Zastrow M, Schulz S. Differentiation of opioid drug effects by hierarchical multi-site phosphorylation. Mol Pharmacol. 2013 Mar;83(3):633-9. doi: 10.1124/mol.112.082875. Epub 2012 Dec 13. PubMed PMID: 23239825; PubMed Central PMCID: PMC3583494.

Schulz S, Mayer D, Pfeiffer M, Stumm R, Koch T, Höllt V. Morphine induces terminal micro-opioid receptor desensitization by sustained phosphorylation of serine-375. EMBO J. 2004 Aug 18;23(16):3282-9. Epub 2004 Jul 22. PubMed PMID: 15272312; PubMed Central PMCID: PMC514508.

Pfeiffer M, Koch T, Schröder H, Laugsch M, Höllt V, Schulz S. Heterodimerization of somatostatin and opioid receptors cross-modulates phosphorylation, internalization, and desensitization. J Biol Chem. 2002 May 31;277(22):19762-72. Epub 2002 Mar 14. PubMed PMID: 11896051.

Koch T, Schulz S, Pfeiffer M, Klutzny M, Schröder H, Kahl E, Höllt V. C-terminal splice variants of the mouse mu-opioid receptor differ in morphine-induced internalization and receptor resensitization. J Biol Chem. 2001 Aug 17;276(33):31408-14. Epub 2001 May 18. PubMed PMID: 11359768.

Selected References Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOPreceptor... read more »
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Epitope-Tag Antibodies

Selected References

Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOPreceptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists. Sci Signal. 2019 Mar 26;12(574). pii: eaau8072. doi: 10.1126/scisignal.aau8072. PubMed PMID: 30914485; PubMed Central PMCID: PMC6934085.

Miess E, Gondin AB, Yousuf A, Steinborn R, Mösslein N, Yang Y, Göldner M, Ruland JG, Bünemann M, Krasel C, Christie MJ, Halls ML, Schulz S, Canals M. Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization. Sci Signal. 2018 Jul 17;11(539). pii: eaas9609. doi: 10.1126/scisignal.aas9609. PubMed PMID: 30018083.

Just S, Illing S, Trester-Zedlitz M, Lau EK, Kotowski SJ, Miess E, Mann A, Doll C, Trinidad JC, Burlingame AL, von Zastrow M, Schulz S. Differentiation of opioid drug effects by hierarchical multi-site phosphorylation. Mol Pharmacol. 2013 Mar;83(3):633-9. doi: 10.1124/mol.112.082875. Epub 2012 Dec 13. PubMed PMID: 23239825; PubMed Central PMCID: PMC3583494.

Schulz S, Mayer D, Pfeiffer M, Stumm R, Koch T, Höllt V. Morphine induces terminal micro-opioid receptor desensitization by sustained phosphorylation of serine-375. EMBO J. 2004 Aug 18;23(16):3282-9. Epub 2004 Jul 22. PubMed PMID: 15272312; PubMed Central PMCID: PMC514508.

Pfeiffer M, Koch T, Schröder H, Laugsch M, Höllt V, Schulz S. Heterodimerization of somatostatin and opioid receptors cross-modulates phosphorylation, internalization, and desensitization. J Biol Chem. 2002 May 31;277(22):19762-72. Epub 2002 Mar 14. PubMed PMID: 11896051.

Koch T, Schulz S, Pfeiffer M, Klutzny M, Schröder H, Kahl E, Höllt V. C-terminal splice variants of the mouse mu-opioid receptor differ in morphine-induced internalization and receptor resensitization. J Biol Chem. 2001 Aug 17;276(33):31408-14. Epub 2001 May 18. PubMed PMID: 11359768.

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