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- Order number: 7TM0040N-IC
- Content: 100 µl
- Host: Rabbit
The BB3 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Bombesin Receptor 3. It can be used to detect BB3 in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.
| Alternative Names | BB3, BRS3, Bombesin Receptor 3 |
| IUPHAR Target ID | 40 |
| UniProt ID | P32247 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC) | not tested |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide with sequence EISVTSFTGCSVKQAEDRF corresponds to amino acids 381-399 of human BB3 |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Immunohistochemical identification of Bombesin receptor 3 in human ovarian carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-BB3 (Bombesin receptor 3) antibody (7TM0040N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, BB3 receptors were detected at the plasma membrane of nearly all cells in human ovarian carcinoma.
