pT359/pS360-BB2 (phospho-Bombesin Receptor 2 Antibody)

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Preview: Agonist-induced Threonine359/Serine360 phosphorylation of the Bombesin Receptor 2

Preview: Analysis of dose-dependent Bombesin Receptor 2 phosphorylation using two phosphosite-specific antibodies

Agonist-induced Threonine359/Serine360 phosphorylation of the Bombesin Receptor 2

Analysis of dose-dependent Bombesin Receptor 2 phosphorylation using two phosphosite-specific antibodies

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Order number: 7TM0039B
Content: 100 µl
Host: Rabbit

Threonine359/Serine360 (T359/S360) is major phosphorylation site of the Bombesin Receptor 2... more

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Threonine359/Serine360 (T359/S360) is major phosphorylation site of the Bombesin Receptor 2 (BB2). The pT359/pS360-BB2 antibody detects phosphorylation in response to agonists. T359/S360 phosphorylation is likely to be involved in efficient ligand sequestration by BB2.

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Alternative Names BB2, GRPR, Bombesin Receptor 2, Gastrin-releasing Peptide... more

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Alternative Names	BB2, GRPR, Bombesin Receptor 2, Gastrin-releasing Peptide Receptor
IUPHAR Target ID	39
UniProt ID	P30550
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human BB2 around the phosphorylation site of Thr359/Ser360.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine359/Serine360 phosphorylation of the Bombesin Receptor 2.... more

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Figure 1. Agonist-induced Threonine359/Serine360 phosphorylation of the Bombesin Receptor 2. Upper panel, HEK293 cells stably expressing the Bombesin receptor 2 (BB2) were either not exposed or exposed to 100 nM Bombesin for 30 minutes. Cells were lysed and immunoblotted with the anti-pT359/pS360-BB2 antibody (7TM0039A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-BB2 antibody (7TM0039N) to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Bombesin Receptor 2 phosphorylation using two phosphosite-specific antibodies. Upper two panels, HEK293 cells stably expressing the Bombesin Receptor 2 (BB2) were either not exposed or exposed to increasing concentrations of Bombesin ranging from 1 nM to 10 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pT359/pS360-BB2 antibody (7TM0039A) or anti-pS363/pS364-BB2 antibody (7TM0039B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-BB2 antibody (7TM0039N) at a dilution of 1:1000 to confirm equal loading of the gel.

Be the first to analyze the ACKR2 phosphorylation using our anti-pS348/pS350-ACKR2... more

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Be the first to analyze the ACKR2 phosphorylation using our anti-pS348/pS350-ACKR2 antibody and let us know.

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