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Phosphorylation of intracellular serine and threonine residues is the most important post translational modification of G protein-coupled receptors (GPCRs) also called heptahelical or seven transmembrane receptors (7TMR). After agonist exposure, these receptors acquire an active conformation, which is recognized by a family of highly specialized GPCR kinases (GRKs). Agonist-driven phosphorylation by GRKs regulates acute receptor desensitization, arrestin recruitment, internalization, post-activation signaling, long-term tolerance and drug addiction. Phosphosite-specific 7TM antibodies are designed to specifically detect agonist-activated GPCRs. In fact, recent work shows that ligand profiling using phosphosite-specific 7TM antibodies provides valuble information on ligand bias beyond that obtained with conventional ß-arrestin recruitment assays. Phosphosite-specific 7TM antibodies are novel tools for GPCR research that can be used to:

  • profile agonist properties of novel GPCR ligands
  • decipher the phosphorylation barcode of GPCRs
  • determine the spatial and temporal dynamics of receptor phosphorylation
  • identify relevant kinases and phosphatases for GPCR phosphorylation and dephosphoryation

Lifecycle3

Schematic representation of the G protein-coupled receptor phosphorylation / dephosphorylation cycle. GRK, G protein-coupled receptor kinase; PKC, protein kinase C; cPP1, catalytic subunit of protein phosphatase 1; R*, activated GPCR; CCP, clathrin-coated pit. 

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Agonist-induced Threonine358/Threonrine361 phosphorylation of the δ-Opioid receptor
pT358/pT361-DOP (phospho-∂-Opioid Receptor...
Threonine358/Threonine361 (T358/T361) is the primary phosphorylation site in a hierarchical phosphorylation cascade of the delta-opioid receptor (DOP). The pT358/T361-DOP antibody detects phosphorylation in response to high- and...
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Agonist-induced Threonine361/Serine363 phosphorylation of the δ-Opioid receptor
pT361/pS363-DOP (phospho-∂-Opioid Receptor...
Threonine361/Serine363 (T361/S363) is the primary phosphorylation site in a hierarchical phosphorylation cascade of the delta-opioid receptor (DOP). The pT361/pS363-DOP antibody detects phosphorylation in response to high- and...
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Agonist-induced Serine363 phosphorylation of the δ-Opioid receptor.
pS363-DOP (phospho-∂-Opioid Receptor Antibody)
Serine363 (S363) is the primary phosphorylation site in a hierarchical phosphorylation cascade of the delta-opioid receptor (DOP). The pS363-DOP antibody detects phosphorylation in response to high- and low-efficacy agonists but not...
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Validation of the δ-Opioid Receptor in transfected HEK293 cells
DOP (non-phospho) ∂-Opioid Receptor Antibody
The non-phospho-∂-opioid receptor antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human DOP. It can be used to detect total DOP receptors in Western blots independent of phosphorylation.
$ 250.00 *
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Agonist-induced Serine326 phosphorylation of the Cannabinoid Receptor 2
pS326-CB2 (phospho-Cannabinoid Receptor 2...
Serine326 (S326) is major phosphorylation site of the Cannabinoid Receptor 2 (CB2). The pS326-CB2 antibody detects phosphorylation in response to agonists. S326 phosphorylation is likely to be involved in efficient ligand sequestration...
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pS335/pS336-CB2 (phospho-Cannabinoid Receptor 2 Antibody)
pS335/pS336-CB2 (phospho-Cannabinoid Receptor 2...
Serine335/Serine336 (S335/S336) is major phosphorylation site of the Cannabinoid Receptor 2 (CB2). The pS335/pS336-CB2 antibody detects phosphorylation in response to agonists. S335/S336 phosphorylation is likely to be involved in...
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Agonist-induced Threonine338/Threonine340 phosphorylation of the Cannabinoid Receptor 2
pT338/pT340-CB2 (phospho-Cannabinoid Receptor 2...
Threonine338/Threonine340 (T338/T340) is major phosphorylation site of the Cannabinoid Receptor 2 (CB2). The pT338/pT340-CB2 antibody detects phosphorylation in response to agonists. T338/T340 phosphorylation is likely to be involved in...
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pT442/pS343-GIPR (phospho-Gastric Inhibitory Polypeptide Receptor Antibody)
pT442/pS343-GIPR (phospho-Gastric Inhibitory...
Threonine442/Serine443 (T442/S443) is major phosphorylation site of human GIP receptor. The pT442/pS443-GIPR antibody detects phosphorylation in response to agonists. T442/S443 phosphorylation is likely to be involved in efficient ligand...
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Agonist-induced Serine594/Threonine597/Serine599 phosphorylation of the SMO Receptor
pS594/pT597/pS599-SMO (phospho-SMO Receptor...
Serine594/Threonine597/Serine599 (S594/S597/S599) is major GRK2 phosphorylation site of the SMO receptor. The pS594/pT597/pS599-SMO antibody detects phosphorylation in response to agonists. The pS594/pT597/pS599-SMO antibody can be used...
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pT358/pS360-GPR132 (phospho-GPR132 Antibody)
pT358/pS360-GPR132 (phospho-GPR132 Antibody)
Threonine339/Serine341 (T339/S341) is major phosphorylation site of human GPR132 receptor. The pT339/pS341-GPR132 antibody detects phosphorylation in response to agonists. T339/S341 phosphorylation is likely to be involved in efficient...
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pT344/pS349-GPR132 (phospho-GPR132 Antibody)
pT344/pS349-GPR132 (phospho-GPR132 Antibody)
Threonine339/Serine341 (T339/S341) is major phosphorylation site of human GPR132 receptor. The pT339/pS341-GPR132 antibody detects phosphorylation in response to agonists. T339/S341 phosphorylation is likely to be involved in efficient...
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pT339/pS341-GPR132 (phospho-GPR132 Antibody)
pT339/pS341-GPR132 (phospho-GPR132 Antibody)
Threonine339/Serine341 (T339/S341) is major phosphorylation site of human GPR132 receptor. The pT339/pS341-GPR132 antibody detects phosphorylation in response to agonists. T339/S341 phosphorylation is likely to be involved in efficient...
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pS335/pS337-S1P3 (phospho-Sphingosine 1-Phosphate Receptor 3 Antibody)
pS335/pS337-S1P3 (phospho-Sphingosine...
Serine351/Serine353 (S351/S353) is major phosphorylation site of the Sphingosine 1-Phosphate Receptor 1 (S1P1). The pS351/pS353-S1P1 antibody detects phosphorylation in response to agonists. S351/S353 phosphorylation is likely to be...
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pT342pS343-S1P2 (phospho-Sphingosine 1-Phosphate Receptor 2 Antibody)
pT342pS343-S1P2 (phospho-Sphingosine...
Serine351/Serine353 (S351/S353) is major phosphorylation site of the Sphingosine 1-Phosphate Receptor 1 (S1P1). The pS351/pS353-S1P1 antibody detects phosphorylation in response to agonists. S351/S353 phosphorylation is likely to be...
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pS330/pS331-S1P2 (phospho-Sphingosine 1-Phosphate Receptor 2 Antibody)
pS330/pS331-S1P2 (phospho-Sphingosine...
Serine351/Serine353 (S351/S353) is major phosphorylation site of the Sphingosine 1-Phosphate Receptor 1 (S1P1). The pS351/pS353-S1P1 antibody detects phosphorylation in response to agonists. S351/S353 phosphorylation is likely to be...
$ 350.00 *
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pS358/pS359-S1P1 (phospho-Sphingosine 1-Phosphate Receptor 1 Antibody)
pS358/pS359-S1P1 (phospho-Sphingosine...
Serine351/Serine353 (S351/S353) is major phosphorylation site of the Sphingosine 1-Phosphate Receptor 1 (S1P1). The pS351/pS353-S1P1 antibody detects phosphorylation in response to agonists. S351/S353 phosphorylation is likely to be...
$ 350.00 *
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1 From 16

For further reading refer to:

Kliewer A, Reinscheid RK, Schulz S. Emerging Paradigms of G Protein-Coupled Receptor Dephosphorylation. Trends Pharmacol Sci. 2017 Jul;38(7):621-636. doi:10.1016/j.tips.2017.04.002. Epub 2017 May 4. Review. PubMed PMID: 28478994.

Miess E, Gondin AB, Yousuf A, Steinborn R, Mösslein N, Yang Y, Göldner M, Ruland JG, Bünemann M, Krasel C, Christie MJ, Halls ML, Schulz S, Canals M. Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization. Sci Signal. 2018 Jul 17;11(539). pii: eaas9609. doi: 10.1126/scisignal.aas9609. PubMed PMID: 30018083.

Kliewer A, Schmiedel F, Sianati S, Bailey A, Bateman JT, Levitt ES, Williams JT, Christie MJ, Schulz S. Phosphorylation-deficient G-protein-biased μ-opioid receptors improve analgesia and diminish tolerance but worsen opioid side effects. Nat Commun. 2019 Jan 21;10(1):367. doi: 10.1038/s41467-018-08162-1. PubMed PMID: 30664663; PubMed Central PMCID: PMC6341117.

Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOP receptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists. Sci Signal. 2019 Mar 26;12(574). pii: eaau8072. doi: 10.1126/scisignal.aau8072. PubMed PMID: 30914485; PubMed Central PMCID: PMC6934085.

Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049. PubMed PMID: 30726732.

Glück L, Loktev A, Moulédous L, Mollereau C, Law PY, Schulz S. Loss of morphine reward and dependence in mice lacking G protein-coupled receptor kinase 5. Biol Psychiatry. 2014 Nov 15;76(10):767-74. doi: 10.1016/j.biopsych.2014.01.021. Epub 2014 Feb 3. PubMed PMID: 24629717; PubMed Central PMCID: PMC4119866.

For further reading refer to: Kliewer A, Reinscheid RK, Schulz S. Emerging Paradigms of G Protein-Coupled Receptor Dephosphorylation. Trends Pharmacol Sci. 2017 Jul;38(7):621-636.... read more »
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For further reading refer to:

Kliewer A, Reinscheid RK, Schulz S. Emerging Paradigms of G Protein-Coupled Receptor Dephosphorylation. Trends Pharmacol Sci. 2017 Jul;38(7):621-636. doi:10.1016/j.tips.2017.04.002. Epub 2017 May 4. Review. PubMed PMID: 28478994.

Miess E, Gondin AB, Yousuf A, Steinborn R, Mösslein N, Yang Y, Göldner M, Ruland JG, Bünemann M, Krasel C, Christie MJ, Halls ML, Schulz S, Canals M. Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization. Sci Signal. 2018 Jul 17;11(539). pii: eaas9609. doi: 10.1126/scisignal.aas9609. PubMed PMID: 30018083.

Kliewer A, Schmiedel F, Sianati S, Bailey A, Bateman JT, Levitt ES, Williams JT, Christie MJ, Schulz S. Phosphorylation-deficient G-protein-biased μ-opioid receptors improve analgesia and diminish tolerance but worsen opioid side effects. Nat Commun. 2019 Jan 21;10(1):367. doi: 10.1038/s41467-018-08162-1. PubMed PMID: 30664663; PubMed Central PMCID: PMC6341117.

Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOP receptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists. Sci Signal. 2019 Mar 26;12(574). pii: eaau8072. doi: 10.1126/scisignal.aau8072. PubMed PMID: 30914485; PubMed Central PMCID: PMC6934085.

Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049. PubMed PMID: 30726732.

Glück L, Loktev A, Moulédous L, Mollereau C, Law PY, Schulz S. Loss of morphine reward and dependence in mice lacking G protein-coupled receptor kinase 5. Biol Psychiatry. 2014 Nov 15;76(10):767-74. doi: 10.1016/j.biopsych.2014.01.021. Epub 2014 Feb 3. PubMed PMID: 24629717; PubMed Central PMCID: PMC4119866.

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