Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 Antibodies
The atypical chemokine receptor 3 (ACKR3, previously called CXC chemokine receptor 7) is a member of the heptahelical receptor superfamily that sequesters its ligands CXCL11 and CXCL12 by highly efficient internalization. While ACKR3 does not engage heterotrimeric G proteins, it regulates the amount of ligand available for CXCR4. ACKR3 and CXCR4 cooperate in the regulation of many chemokine-dependent processes such as directed cell migration, immune homeostasis, inflammation and cancer. Efficient ligand sequestration by ACKR3 critically depends on GRK2 and GRK5 mediated phosphorylation of carboxyl-terminal serine350/threonine352 (pS350/pT352-ACKR3/CXCR7) and serine355/serine360 (pS355/pS360-ACKR3/CXCR7) but not on β-arrestin recruitment. This phosphorylation motif is highly conserved across a variety of species including mouse, rat and human. For more information on ACKR3 pharmacology please refer to the IUPHAR database. For further reading refer to:
Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049. PubMed PMID: 30726732.
Sánchez-Alcañiz JA, Haege S, Mueller W, Pla R, Mackay F, Schulz S, López-Bendito G, Stumm R, Marín O. Cxcr7 controls neuronal migration by regulating chemokine responsiveness. Neuron. 2011 Jan 13;69(1):77-90. doi: 10.1016/j.neuron.2010.12.006. PubMed PMID: 21220100.
Hoffmann F, Müller W, Schütz D, Penfold ME, Wong YH, Schulz S, Stumm R. Rapid uptake and degradation of CXCL12 depend on CXCR7 carboxyl-terminal serine/threonine residues. J Biol Chem. 2012 Aug 17;287(34):28362-77. doi: 10.1074/jbc.M111.335679. Epub 2012 Jun 26. PubMed PMID: 22736769; PubMed Central PMCID: PMC3436560.