LPA3 (non-phospho), Lysophosphatidic Acid Receptor 3 Antibody

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Order number: 7TM0274N
Content: 100 µl
Host: Rabbit

The non-phospho-LPA3 receptor antibody is directed against the distal end of the... more

The non-phospho-LPA3 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human LPA3. It also detects LPA3 in cultured cells and tissue sections by immunohistochemistry. It can be used to detect total LPA3 receptors in Western blots independent of phosphorylation. The LPA3 antibody can also be used to isolate and enrich LPA3 receptors from tissue lysates.

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Alternative Names CD363, Edg1, endothelial differentiation G... more

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Alternative Names	CD363, Edg1, endothelial differentiation G protein-coupled receptor 1 Receptor
IUPHAR Target ID	275
Uniprot ID	P21453
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to c-terminal end of human S1P1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the S1P1 Receptor in transfected HEK293 cells. Native HEK293 cells... more

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Figure 1. Validation of the S1P1 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the S1P1 Receptor (S1P1) were lysed and immunoblotted with the phosphorylation-independent anti-S1P1 antibody (7TM0275N) at a dilution of 1:100.

Figure 2. Immunohistochemical identification of S1P1 Receptor in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-S1P1 (non-phospho-Sphingosine 1-Phosphate Receptor 1) antibody (7TM0275N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 3. Immunohistochemical identification of S1P1 Receptor in human kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-S1P1 (non-phospho-Sphingosine 1-Phosphate Receptor 1) antibody (7TM0275N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 4. Immunohistochemical varification of S1P1 Receptor antibody in human spleen. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-S1P1 (non-phospho-S1P1 Receptor) antibody (7TM0275N-IC). Sections were then incubated with anti-S1P1 (non-phospho-S1P1 Receptor) antibody (7TM0275N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation S1P1 receptors were uniformly detected at the plasma membrane of nearly all cells.

Figure 5. Immunohistochemical varification of S1P1 Receptor antibody in human kidney. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-S1P1 (non-phospho-S1P1 Receptor) antibody (7TM0275N-IC). Sections were then incubated with anti-S1P1 (non-phospho-S1P1 Receptor) antibody (7TM0275N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation S1P1 receptors were uniformly detected at the plasma membrane of nearly all cells.