GRK5/6 Inhibitor (Cmpd 18)

CAS No.: 2677786-61-5

Molecular Weight: 438.91

Figure 1. Immunohistochemical identification of G Protein-coupled Receptor 17 in oligodendrocytes. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR17 (G Protein-coupled Receptor 17) antibody (7TM0088N-ICGP) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR17 receptors were detected at the plasma membrane of cells.

Figure 2. Inhibition of agonist-induced pT360/pS364-β2 adrenergic receptor (β2) phosphorylation by GRK5/6 inhibitor (Cmpd 18).

Figure 3. Indentification of G Protein-coupled Receptor 17 in mouse tissues. Native mouse tissues were lysed and GPCR receptors were enriched by using WGA-beads (see 7TM Western Blot Protocol). Extracted proteins were immunoblotted with the phosphorylation-independent anti-GPR17 antibody (7TM0088N-ICGP) at a dilution of 1:1000.