D2 (IHC-grade), Dopamine Receptor 2 Antibody

Figure 1. Immunohistochemical identification of Dopamine Receptor 2 in striatum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D1 (Dopamine Receptor 1) antibody (7TM0214N-IC) at a dilution of 1:100 (left panel) or incubated with anti-D2 (Dopamine Receptor 2) antibody (7TM0215N-IC) at a dilution of 1:100 (right panel). Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 2. Immunohistochemical varification of Dopamine Receptor 2 antibody in striatum. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-D2 (non-phospho-Dopamine Receptor 2) antibody (7TM0215N-IC). Sections were then incubated with anti-D2 (non-phospho-Dopamine Receptor 2) antibody (7TM0215N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation S1P1 receptors were uniformly detected at the plasma membrane of nearly all cells.

Figure 3. Validation of the Dopamine Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Dopamine Receptor 2 (D2) were lysed and immunoblotted with the phosphorylation-independent anti-D2 antibody (7TM0215N-WB) at a dilution of 1:1000.

Mann A, Keen AC, Mark H, Dasgupta P, Javitch JA, Canals M, Schulz S, Robert Lane J. New phosphosite-specific antibodies to unravel the role of GRK phosphorylation in dopamine D2 receptor regulation and signaling. Sci Rep. 2021 Apr 15;11(1):8288. doi: 10.1038/s41598-021-87417-2. PMID: 33859231; PMCID: PMC8050214.