pS317/pT318-D2 (phospho-Dopamine Receptor 2 Antibody)

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pS317/pT318-D2 (phospho-Dopamine Receptor 2 Antibody)

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Order number: 7TM0215A
Content: 100 µl
Host: Rabbit

Serine317/threonine318 (S317/T318) is a major phosphorylation site of the D2 dopamine receptor.... more

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Serine317/threonine318 (S317/T318) is a major phosphorylation site of the D2 dopamine receptor. The pS317/pT318-D2 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. S317/T318 phosphorylation is a key regulator of D2 desensitization, β-arrestin recruitment and internalization.

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Alternative Names D2, D2R, Dopamine Receptor 2 IUPHAR Target ID... more

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Alternative Names	D2, D2R, Dopamine Receptor 2
IUPHAR Target ID	215
UniProt ID	P14416
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human D2 around the phosphorylation site of Ser317/Thr318
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine317/Threonine318 phosphorylation of the Dopamine Receptor 2.... more

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Figure 1. Agonist-induced Serine317/Threonine318 phosphorylation of the Dopamine Receptor 2. Upper panel, HEK293 cells stably expressing the Dopamine Receptor 2 (D2) were either not exposed or exposed to 10 μM Quinprirole or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pS317/pT318-D2 antibody (7TM0215A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-D2 antibody (7TM0215N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Dopamine Receptor 2 phosphorylation. Upper panel, HEK293 cells stably expressing the Dopamine Receptor 2 (D2) were either not exposed or exposed to increasing concentrations of Quinpirole ranging from 1 nM to 10 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS317/pT318-D2 antibody (7TM0215A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-D2 antibody (7TM0215N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Mann A, Keen AC, Mark H, Dasgupta P, Javitch JA, Canals M, Schulz S, Robert Lane J. New... more

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Mann A, Keen AC, Mark H, Dasgupta P, Javitch JA, Canals M, Schulz S, Robert Lane J. New phosphosite-specific antibodies to unravel the role of GRK phosphorylation in dopamine D2 receptor regulation and signaling. Sci Rep. 2021 Apr 15;11(1):8288. doi: 10.1038/s41598-021-87417-2. PMID: 33859231; PMCID: PMC8050214.

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