Premium Phosphosite-Specific 7TM Antibodies
Your Resource for GPCR Research
Select Your Country of Delivery below

Premium Phosphosite-Specific 7TM Antibodies

Phosphorylation of intracellular serine and threonine residues is the most important post translational modification of G protein-coupled receptors (GPCRs) also called heptahelical or seven transmembrane receptors (7TMR). After agonist exposure, these receptors acquire an active conformation, which is recognized by a family of highly specialized GPCR kinases (GRKs). Agonist-driven phosphorylation by GRKs regulates acute receptor desensitization, arrestin recruitment, internalization, post-activation signaling, long-term tolerance and drug addiction. Phosphosite-specific 7TM antibodies are designed to specifically detect agonist-activated GPCRs. In fact, recent work shows that ligand profiling using phosphosite-specific 7TM antibodies provides valuble information on ligand bias beyond that obtained with conventional ß-arrestin recruitment assays. Phosphosite-specific 7TM antibodies are novel tools for GPCR research that can be used to:

  • profile agonist properties of novel GPCR ligands
  • decipher the phosphorylation barcode of GPCRs
  • determine the spatial and temporal dynamics of receptor phosphorylation
  • identify relevant kinases and phosphatases for GPCR phosphorylation and dephosphoryation

Lifecycle3

Schematic representation of the G protein-coupled receptor phosphorylation / dephosphorylation cycle. GRK, G protein-coupled receptor kinase; PKC, protein kinase C; cPP1, catalytic subunit of protein phosphatase 1; R*, activated GPCR; CCP, clathrin-coated pit. 

Close filters
24 From 38
No results were found for the filter!
NEW
Validation of the Neuropeptide FF Receptor 1 in transfected HEK293 cells
NPFF1 (non-phospho), Neuropeptide FF Receptor 1...
The NPFF1 antibody is directed against the distal end of the carboxyl-terminal tail of human NPFF1. It detects selectively the NPFF1. It can be used to detect total NPFF1 receptors in Western blots independent of phosphorylation. The...
$ 375.00 *
Details
NEW
Validation of the EP3 Prostanoid Receptor in transfected HEK293 cells
EP3 (non-phospho), EP3 Prostanoid Receptor...
The non-phospho-EP3 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human EP3. It can be used to detect total EP3 receptors in Western blots independent of phosphorylation.The EP3 antibody can also...
$ 375.00 *
Details
NEW
Agonist-induced Threonine315 phosphorylation of the GPR139 Receptor
pT315-GPR139 (phospho-GPR139 Antibody)
Threonine315 (T315) is major phosphorylation site of the GPR139 Receptor (GPR139). The pT315-GPR139 antibody detects phosphorylation in response to agonists. T315 phosphorylation is likely to be involved in efficient ligand sequestration.
$ 375.00 *
Details
NEW
Agonist-induced Serine323/Serine324 phosphorylation of the GPR139 Receptor
pS323/pS324-GPR139 (phospho-GPR139 Antibody)
Serine323/Serine324 (S323/S324) is major phosphorylation site of the GPR139 Receptor (GPR139). The pS323/pS324-GPR139 antibody detects phosphorylation in response to agonists. S323/S324 phosphorylation is likely to be involved in...
$ 375.00 *
Details
NEW
Validation of the mouse S1P5 Receptor in transfected HEK293 cells
mS1P5 (non-phospho), Mouse Sphingosine...
The non-phospho-mS1P5 receptor antibody is directed against the distal end of the carboxyl-terminal tail of mouseS1P5. It can be used to detect total mS1P5 receptors in Western blots independent of phosphorylation. The mS1P5 antibody can...
$ 375.00 *
Details
NEW
Validation of the mouse S1P5 Receptor in transfected HEK293 cells
mS1P5 (GP-non-phospho), Mouse Sphingosine...
The non-phospho-mS1P5 receptor antibody is directed against the distal end of the carboxyl-terminal tail of mouseS1P5. It can be used to detect total mS1P5 receptors in Western blots independent of phosphorylation. The mS1P5 antibody can...
$ 375.00 *
Details
NEW
Validation of the GPR88 Receptor in transfected HEK293 cells
GPR88 (non-phospho), G protein-coupled...
The non-phospho-GPR88 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human GPR88. It can be used to detect total GPR88 receptors in Western blots independent of phosphorylation. The GPR88 antibody...
$ 375.00 *
Details
NEW
Agonist-induced Serine346/Serine349 phosphorylation of GPR88-Receptor
pS346/pS349-GPR88 (phospho-GPR88 Antibody)
Serine346/Serine349 (S346/S349) is a major phosphorylation site of the human GPR88 receptor. The pS346/pS349-GPR88 antibody detects phosphorylation in response to high-efficacy agonists. S346/S349 phosphorylation is a key regulator of...
$ 375.00 *
Details
NEW
Agonist-induced Serine371 phosphorylation of GPR88-Receptor.
pS371-GPR88 (phospho-GPR88 Antibody)
Serine371 (S371) is a major phosphorylation site of the human GPR88 receptor. The pS371-GPR88 antibody detects phosphorylation in response to high-efficacy agonists. S371 phosphorylation is a key regulator of human GPR88 desensitization,...
$ 375.00 *
Details
NEW
Agonist-induced Threonine377 phosphorylation of GPR88-Receptor
pT377-GPR88 (phospho-GPR88 Antibody)
Threonine377 (T377) is a major phosphorylation site of the human GPR88 receptor. The pT377-GPR88 antibody detects phosphorylation in response to high-efficacy agonists. T377 phosphorylation is a key regulator of human GPR88...
$ 375.00 *
Details
NEW
Immunohistochemical varification of S1P2 Receptor antibody in human liver.
S1P2 (IHC-grade), Sphingosine 1-Phosphate...
The non-phospho-S1P2 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human S1P2. It also detects S1P2 in cultured cells and tissue sections by immunohistochemistry. It can be used to detect total...
$ 375.00 *
Details
NEW
Validation of the S1P2 Receptor in transfected HEK293 cells.
S1P2 (non-phospho), Sphingosine 1-Phosphate...
The non-phospho-S1P2 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human S1P2. It also detects S1P2 in cultured cells and tissue sections by immunohistochemistry. It can be used to detect total...
$ 375.00 *
Details
NEW
Validation of the S1P4 Receptor in transfected HEK293 cells
S1P4 (non-phospho), Sphingosine 1-Phosphate...
The non-phospho-S1P4 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human S1P4. It can be used to detect total S1P4 receptors in Western blots independent of phosphorylation. The S1P4 antibody can...
$ 375.00 *
Details
NEW
Validation of the PAF Receptor in transfected HEK293 cells
PAF (non-phospho), Platelet-Activating Factor...
The non-phospho-PAF receptor antibody is directed against the distal end of the carboxyl-terminal tail of human PAF. It can be used to detect total PAF receptors in Western blots independent of phosphorylation. The PAF antibody can also...
$ 375.00 *
Details
Rabbit Monoclonal
KD-Validated
NEW
Validation of the Somatostatin Receptor 3 in transfected HEK293 cells.
SST3 [UMB5] (RM-non-phospho), Somatostatin...
The non-phospho-SST3 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human SST3. In can be used to detect total SST3 receptors in Western blots independent of phosphorylation. The non-phospho-SST3...
$ 495.00 *
Details
Rabbit Monoclonal
KD-Validated
NEW
Immunohistochemical identification of Somatostatin Receptor 3 in human growth-hormone-producing pituitary adenoma tissue.
SST3 [UMB5] (RM-IHC-grade), Somatostatin...
The SST3 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Somatostatin Receptor 3. In can be used to detect total SST3 receptors in Western blots independent of phosphorylation. The SST3...
$ 495.00 *
Details
24 From 38

For further reading refer to:

Kliewer A, Reinscheid RK, Schulz S. Emerging Paradigms of G Protein-Coupled Receptor Dephosphorylation. Trends Pharmacol Sci. 2017 Jul;38(7):621-636. doi:10.1016/j.tips.2017.04.002. Epub 2017 May 4. Review. PubMed PMID: 28478994.

Miess E, Gondin AB, Yousuf A, Steinborn R, Mösslein N, Yang Y, Göldner M, Ruland JG, Bünemann M, Krasel C, Christie MJ, Halls ML, Schulz S, Canals M. Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization. Sci Signal. 2018 Jul 17;11(539). pii: eaas9609. doi: 10.1126/scisignal.aas9609. PubMed PMID: 30018083.

Kliewer A, Schmiedel F, Sianati S, Bailey A, Bateman JT, Levitt ES, Williams JT, Christie MJ, Schulz S. Phosphorylation-deficient G-protein-biased μ-opioid receptors improve analgesia and diminish tolerance but worsen opioid side effects. Nat Commun. 2019 Jan 21;10(1):367. doi: 10.1038/s41467-018-08162-1. PubMed PMID: 30664663; PubMed Central PMCID: PMC6341117.

Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOP receptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists. Sci Signal. 2019 Mar 26;12(574). pii: eaau8072. doi: 10.1126/scisignal.aau8072. PubMed PMID: 30914485; PubMed Central PMCID: PMC6934085.

Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049. PubMed PMID: 30726732.

Glück L, Loktev A, Moulédous L, Mollereau C, Law PY, Schulz S. Loss of morphine reward and dependence in mice lacking G protein-coupled receptor kinase 5. Biol Psychiatry. 2014 Nov 15;76(10):767-74. doi: 10.1016/j.biopsych.2014.01.021. Epub 2014 Feb 3. PubMed PMID: 24629717; PubMed Central PMCID: PMC4119866.

For further reading refer to: Kliewer A, Reinscheid RK, Schulz S. Emerging Paradigms of G Protein-Coupled Receptor Dephosphorylation. Trends Pharmacol Sci. 2017 Jul;38(7):621-636.... read more »
Close window
Premium Phosphosite-Specific 7TM Antibodies

For further reading refer to:

Kliewer A, Reinscheid RK, Schulz S. Emerging Paradigms of G Protein-Coupled Receptor Dephosphorylation. Trends Pharmacol Sci. 2017 Jul;38(7):621-636. doi:10.1016/j.tips.2017.04.002. Epub 2017 May 4. Review. PubMed PMID: 28478994.

Miess E, Gondin AB, Yousuf A, Steinborn R, Mösslein N, Yang Y, Göldner M, Ruland JG, Bünemann M, Krasel C, Christie MJ, Halls ML, Schulz S, Canals M. Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization. Sci Signal. 2018 Jul 17;11(539). pii: eaas9609. doi: 10.1126/scisignal.aas9609. PubMed PMID: 30018083.

Kliewer A, Schmiedel F, Sianati S, Bailey A, Bateman JT, Levitt ES, Williams JT, Christie MJ, Schulz S. Phosphorylation-deficient G-protein-biased μ-opioid receptors improve analgesia and diminish tolerance but worsen opioid side effects. Nat Commun. 2019 Jan 21;10(1):367. doi: 10.1038/s41467-018-08162-1. PubMed PMID: 30664663; PubMed Central PMCID: PMC6341117.

Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOP receptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists. Sci Signal. 2019 Mar 26;12(574). pii: eaau8072. doi: 10.1126/scisignal.aau8072. PubMed PMID: 30914485; PubMed Central PMCID: PMC6934085.

Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049. PubMed PMID: 30726732.

Glück L, Loktev A, Moulédous L, Mollereau C, Law PY, Schulz S. Loss of morphine reward and dependence in mice lacking G protein-coupled receptor kinase 5. Biol Psychiatry. 2014 Nov 15;76(10):767-74. doi: 10.1016/j.biopsych.2014.01.021. Epub 2014 Feb 3. PubMed PMID: 24629717; PubMed Central PMCID: PMC4119866.

Recently viewed