Premium Phosphosite-Specific 7TM Antibodies
Your Resource for GPCR Research
Select Your Country of Delivery below

Premium Phosphosite-Specific 7TM Antibodies

Phosphorylation of intracellular serine and threonine residues is the most important post translational modification of G protein-coupled receptors (GPCRs) also called heptahelical or seven transmembrane receptors (7TMR). After agonist exposure, these receptors acquire an active conformation, which is recognized by a family of highly specialized GPCR kinases (GRKs). Agonist-driven phosphorylation by GRKs regulates acute receptor desensitization, arrestin recruitment, internalization, post-activation signaling, long-term tolerance and drug addiction. Phosphosite-specific 7TM antibodies are designed to specifically detect agonist-activated GPCRs. In fact, recent work shows that ligand profiling using phosphosite-specific 7TM antibodies provides valuble information on ligand bias beyond that obtained with conventional ß-arrestin recruitment assays. Phosphosite-specific 7TM antibodies are novel tools for GPCR research that can be used to:

  • profile agonist properties of novel GPCR ligands
  • decipher the phosphorylation barcode of GPCRs
  • determine the spatial and temporal dynamics of receptor phosphorylation
  • identify relevant kinases and phosphatases for GPCR phosphorylation and dephosphoryation

Lifecycle3

Schematic representation of the G protein-coupled receptor phosphorylation / dephosphorylation cycle. GRK, G protein-coupled receptor kinase; PKC, protein kinase C; cPP1, catalytic subunit of protein phosphatase 1; R*, activated GPCR; CCP, clathrin-coated pit. 

Close filters
23 From 37
No results were found for the filter!
NEW
Validation of the Tachykinin Receptor 2 in transfected HEK293 cells
NK2 (non-phospho), Tachykinin Receptor 2 Antibody
The NK2 antibody is directed against the distal end of the carboxyl-terminal tail of human Tachykinin Receptor 2. It detects selectively the NK2 Tachykinin Receptor. It can be used to detect total NK2 receptors in Western blots...
$ 375.00 *
Details
NEW
Validation of the MRGPRX3 Receptor in transfected HEK293 cells
MRGPRX3 (non-phospho), MRGPRX3 Mas-related...
The non-phospho-MRGPRX3 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human MRGPRX3. It can be used to detect total MRGPRX3 receptors in Western blots independent of phosphorylation. The MRGPRX3...
$ 375.00 *
Details
NEW
Validation of the GPR161 Receptor in transfected HEK293 cells
GPR161 (non-phospho), G protein-coupled...
The non-phospho-GPR161 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human GPR161. It can be used to detect total GPR161 receptors in Western blots independent of phosphorylation. The GPR161...
$ 375.00 *
Details
NEW
Validation of the Ghrelin Receptor in transfected HEK293 cells
GHSR (non-phospho), Ghrelin Receptor Antibody
The non-phospho-GHSR receptor antibody is directed against the distal end of the carboxyl-terminal tail of human GHSR. It can be used to detect total GHSR receptors in Western blots independent of phosphorylation. The non-phospho-GHSR...
$ 375.00 *
Details
NEW
Validation of the CXCR5 Receptor in transfected HEK293 cells
CXCR5 (non-phospho), CXCR5 Chemokine Receptor...
The non-phospho-CXCR5 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human CXCR5. It can be used to detect total CXCR5 receptors in Western blots independent of phosphorylation.
$ 375.00 *
Details
NEW
Validation of the CXCR2 Receptor in transfected HEK293 cells
CXCR2 (non-phospho), CXCR2 Chemokine Receptor...
The non-phospho-CXCR2 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human CXCR2. It can be used to detect total CXCR2 receptors in Western blots independent of phosphorylation.
$ 375.00 *
Details
NEW
Immunohistochemical identification of Threonine344/Serine347 phosphorylation of NK1 Receptor in striatum
pT344/pS347-NK1 (IHC-grade phospho-Tachykinin...
Threonine344/Serine347 (T344/S347) is a major phosphorylation site of the NK1 receptor. The pT344/pS347-NK1 antibody detects phosphorylation in response to high-efficacy agonists. T344/S347 phosphorylation is a key regulator of NK1...
$ 375.00 *
Details
NEW
Immunohistochemical identification of M1 Muscarinic Acetylcholine Receptor in hippocampus
M1 (IHC-grade), M1 Muscarinic Acetylcholine...
The non-phospho-M1 receptor antibody is directed against the 3rd loop of human M1 Muscarinic Acetylcholine Receptor (M1). It can be used to detect total M1 receptors in Western blots independent of phosphorylation. The non-phospho-M1...
$ 375.00 *
Details
NEW
Agonist-induced Serine355/Serine360 phosphorylation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7
pS355/pS360-ACKR3 (phospho-Atypical Chemokine...
Serine355/Serine360 (S355/S360) is major phosphorylation site of the Atypical Chemokine Receptor 3 (ACKR3, previously called CXCR7). The pS355/pS360-ACKR3 antibody detects phosphorylation in response to agonists. S355/S360...
$ 375.00 *
Details
NEW
Validation of the SUCNR1 Receptor in transfected HEK293 cells
SUCNR1/GPR91 (non-phospho), Succinate Receptor...
The non-phospho-SUCNR1 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human SUCNR1. It can be used to detect total SUCNR1 receptors in Western blots independent of phosphorylation. The SUCNR1...
$ 375.00 *
Details
NEW
Validation of the EP2 Receptor in transfected HEK293 cells
EP2 (non-phospho), EP2 Prostanoid Receptor...
The non-phospho-EP2 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human EP2. It can be used to detect total EP2 receptors in Western blots independent of phosphorylation.The EP2 antibody can also...
$ 375.00 *
Details
NEW
Validation of the EP4 Receptor in transfected HEK293 cells.
EP4 (non-phospho), EP4 Prostanoid Receptor...
The non-phospho-EP4 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human EP4. It can be used to detect total EP4 receptors in Western blots independent of phosphorylation.
$ 375.00 *
Details
NEW
Indentification of EP4 Receptor in mouse tissues
EP4 (GP-non-phospho), EP4 Prostanoid Receptor...
The non-phospho-EP4 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human EP4. It can be used to detect total EP4 receptors in Western blots independent of phosphorylation.
$ 375.00 *
Details
Citations
KO-Validated
Immunohistochemical identification of CXC Chemokine Receptor 4 in human endometrial carcinoma.
CXCR4 (IHC-grade), CXC Chemokine Receptor 4...
The CXCR4 receptor antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human CXCR4. It can be used to detect CXCR4 receptors in Western blots in a phosphorylation-sensitive manner. After agonist...
$ 375.00 *
Details
NEW
CX3CR1 (non-phospho), CX3C Chemokine Receptor 1 Antibody
CX3CR1 (non-phospho), CX3C Chemokine Receptor 1...
The non-phospho-CX3CR1 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human CX3CR1. It can be used to detect total CX3CR1 receptors in Western blots independent of phosphorylation.
$ 375.00 *
Details
NEW
CXCR3 (non-phospho), CXCR3 Chemokine Receptor Antibody
CXCR3 (non-phospho), CXCR3 Chemokine Receptor...
The non-phospho-CXCR3 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human CXCR3. It can be used to detect total CXCR3 receptors in Western blots independent of phosphorylation.
$ 375.00 *
Details
23 From 37

For further reading refer to:

Kliewer A, Reinscheid RK, Schulz S. Emerging Paradigms of G Protein-Coupled Receptor Dephosphorylation. Trends Pharmacol Sci. 2017 Jul;38(7):621-636. doi:10.1016/j.tips.2017.04.002. Epub 2017 May 4. Review. PubMed PMID: 28478994.

Miess E, Gondin AB, Yousuf A, Steinborn R, Mösslein N, Yang Y, Göldner M, Ruland JG, Bünemann M, Krasel C, Christie MJ, Halls ML, Schulz S, Canals M. Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization. Sci Signal. 2018 Jul 17;11(539). pii: eaas9609. doi: 10.1126/scisignal.aas9609. PubMed PMID: 30018083.

Kliewer A, Schmiedel F, Sianati S, Bailey A, Bateman JT, Levitt ES, Williams JT, Christie MJ, Schulz S. Phosphorylation-deficient G-protein-biased μ-opioid receptors improve analgesia and diminish tolerance but worsen opioid side effects. Nat Commun. 2019 Jan 21;10(1):367. doi: 10.1038/s41467-018-08162-1. PubMed PMID: 30664663; PubMed Central PMCID: PMC6341117.

Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOP receptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists. Sci Signal. 2019 Mar 26;12(574). pii: eaau8072. doi: 10.1126/scisignal.aau8072. PubMed PMID: 30914485; PubMed Central PMCID: PMC6934085.

Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049. PubMed PMID: 30726732.

Glück L, Loktev A, Moulédous L, Mollereau C, Law PY, Schulz S. Loss of morphine reward and dependence in mice lacking G protein-coupled receptor kinase 5. Biol Psychiatry. 2014 Nov 15;76(10):767-74. doi: 10.1016/j.biopsych.2014.01.021. Epub 2014 Feb 3. PubMed PMID: 24629717; PubMed Central PMCID: PMC4119866.

For further reading refer to: Kliewer A, Reinscheid RK, Schulz S. Emerging Paradigms of G Protein-Coupled Receptor Dephosphorylation. Trends Pharmacol Sci. 2017 Jul;38(7):621-636.... read more »
Close window
Premium Phosphosite-Specific 7TM Antibodies

For further reading refer to:

Kliewer A, Reinscheid RK, Schulz S. Emerging Paradigms of G Protein-Coupled Receptor Dephosphorylation. Trends Pharmacol Sci. 2017 Jul;38(7):621-636. doi:10.1016/j.tips.2017.04.002. Epub 2017 May 4. Review. PubMed PMID: 28478994.

Miess E, Gondin AB, Yousuf A, Steinborn R, Mösslein N, Yang Y, Göldner M, Ruland JG, Bünemann M, Krasel C, Christie MJ, Halls ML, Schulz S, Canals M. Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization. Sci Signal. 2018 Jul 17;11(539). pii: eaas9609. doi: 10.1126/scisignal.aas9609. PubMed PMID: 30018083.

Kliewer A, Schmiedel F, Sianati S, Bailey A, Bateman JT, Levitt ES, Williams JT, Christie MJ, Schulz S. Phosphorylation-deficient G-protein-biased μ-opioid receptors improve analgesia and diminish tolerance but worsen opioid side effects. Nat Commun. 2019 Jan 21;10(1):367. doi: 10.1038/s41467-018-08162-1. PubMed PMID: 30664663; PubMed Central PMCID: PMC6341117.

Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOP receptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists. Sci Signal. 2019 Mar 26;12(574). pii: eaau8072. doi: 10.1126/scisignal.aau8072. PubMed PMID: 30914485; PubMed Central PMCID: PMC6934085.

Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049. PubMed PMID: 30726732.

Glück L, Loktev A, Moulédous L, Mollereau C, Law PY, Schulz S. Loss of morphine reward and dependence in mice lacking G protein-coupled receptor kinase 5. Biol Psychiatry. 2014 Nov 15;76(10):767-74. doi: 10.1016/j.biopsych.2014.01.021. Epub 2014 Feb 3. PubMed PMID: 24629717; PubMed Central PMCID: PMC4119866.

Recently viewed