A2A (non-phospho) Adenosine 2A Receptor Antibody

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Preview: Validation of the A2A Adenosine Receptor in transfected HEK293 cells

Preview: Immunohistochemical identification of the A2A Adenosine Receptor in spleen

Preview: Immunohistochemical identification of the A2A Adenosine Receptor in human cortex

Validation of the A2A Adenosine Receptor in transfected HEK293 cells

Immunohistochemical identification of the A2A Adenosine Receptor in spleen

Immunohistochemical identification of the A2A Adenosine Receptor in human cortex

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Order number: 7TM0019N
Content: 100 µl
Host: Rabbit

The non-phospho-A2A receptor antibody is directed against the distal end of the... more

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The non-phospho-A2A receptor antibody is directed against the distal end of the carboxyl-terminal tail of human A2A. It can be used to detect total A2A receptors in Western blots independent of phosphorylation. The A2A antibody can also be used to isolate and enrich A2A receptors from tissue lysates. It also detects A2A in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names A2-AR, Adenosine Receptor A2A IUPHAR Target ID... more

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Alternative Names	A2-AR, Adenosine Receptor A2A
IUPHAR Target ID	19
UniProt ID	P29274
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents the carboxyl-terminal tail of human A2A.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the A2A Adenosine Receptor... more

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Figure 1. Validation of the A2A Adenosine Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the A2A Adenosine Receptor (A2A) were lysed and immunoblotted with the anti-A2A antibody (7TM0019N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of the A2A Adenosine Receptor in spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-A2A (A2A Adenosine Receptor) antibody (7TM0019N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 3. Immunohistochemical identification of the A2A Adenosine Receptor in human cortex. Sections were dewaxed, microwaved in citric acid, and incubated with anti-A2A (A2A Adenosine Receptor) antibody (7TM0019N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.