pT361-DOP (phospho-∂-Opioid Receptor Antibody)

Figure 1. Agonist-induced Threonine361 phosphorylation of the δ-Opioid Receptor. Upper panel, HEK293 cells stably expressing the δ-Opioid Receptor (DOP) were either not exposed or exposed to 10 μM DPDPE ([D-Pen2,D-Pen5]-enkephalin) or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT361-DOP antibody (7TM0317A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-DOP antibody to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent δ-Opioid Receptor phosphorylation using two phosphosite-specific antibodies. Upper two panels, HEK293 cells stably expressing the δ-Opioid Receptor (DOP) were either not exposed or exposed to increasing concentrations of selective δ-opioid receptor agonist SNC80 ranging from 1 nM to 10 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pT361-DOP antibody (7TM0317A) or anti-pS363-DOP antibody (7TM0317B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-DOP antibody to confirm equal loading of the gel.

Mann A, Liebetrau S, Klima M, Dasgupta P, Massotte D, Schulz S. Agonist-induced phosphorylation bar code and differential post-activation signaling of the delta opioid receptor revealed by phosphosite-specific antibodies. Sci Rep. 2020 May 22;10(1):8585. doi: 10.1038/s41598-020-65589-7. PMID: 32444688