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VPAC2 (IHC-grade), VIP Receptor 2 Antibody

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KO-Validated
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  • 7TM0372N-IC
  • 100 µl
  • Rabbit
The VPAC2 antibody is directed against the distal end of the carboxyl-terminal tail of mouse,... more

The VPAC2 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human VIP receptor 2. It can be used to detect total VPAC2 receptors in Western blots independent of phosphorylation. The VPAC2 antibody can also be used to isolate and enrich VPAC2 receptors from cell and tissue lysates. It also detects VPAC2 in cultured cells and tissue sections by immunohistochemistry.

  Alternative Names VPAC2, VIP Receptor 2, Vasoactive Intestinal Peptide... more

 

Alternative Names VPAC2, VIP Receptor 2, Vasoactive Intestinal Peptide Receptor 2
IUPHAR Target ID  372
UniProt ID  P41587 (human) P41588 (mouse) P35000 (rat)
Western Blot (WB)  1:1000
Immunocytochemistry (ICC) 1:200
Immunohistochemistry (IHC) 1:100
Species Reactivity   Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide with sequence LQFHRGSRAQSFLQTETSVI corresponds to amino acids 419-438 of VPAC2
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Immunohistochemical identification of VIP Receptor 2 in small intestine. Sections... more

Figure 1. Immunohistochemical identification of VIP Receptor 2 in small intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-VPAC2 (VIP receptor 2) antibody (7TM0372N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, VPAC2 receptors were detected at the plasma membrane of neuroendocrine cells in small intestine.

Figure 2. Immunohistochemical identification of VIP Receptor 2 in breast carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-VPAC2 (VIP receptor 2) antibody (7TM0372N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, VPAC2 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

Figure 3. Immunohistochemical identification of VIP Receptor 2 in lung carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-VPAC2 (VIP receptor 2) antibody (7TM0372N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, VPAC2 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

Figure 4. Immunohistochemical identification of VIP Receptor 2 in pancreatic carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-VPAC2 (VIP receptor 2) antibody (7TM0372N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, VPAC2 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

Figure 5. Validation of the VIP Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the VIP Receptor 2 (VPAC2) were lysed and immunoblotted with the phosphorylation-independent anti-VPAC2 antibody (7TM0372N-IC) at a dilution of 1:1000.

Figure 6. Immunocytochemical identification of VIP Receptor 2 in HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the VIP receptor 2 (VPAC2) were immunocytochemically stained with anti-VPAC2 (VIP receptor 2) antibody (7TM0372N-IC) at a dilution of 1:200. Note, VPAC2 receptors were confined to the plasma membrane in transfected cells.

Figure 7. Western blot analysis of VIP Receptor 2 in mouse tissues in vivo. Tissues from VPAC2-deficient mice (VPAC2-KO) and their wild-type littermates (VPAC2-WT) were dissected, homogenated and immunoblotted using anti-VPAC2 (VIP receptor 2) antibody (7TM0372N-IC) at a dilution of 1:1000. Note, absence of VPAC2 receptors in stomach and colon in VPAC2-KO but not in VPAC2-WT mice.

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