pS409/pS410-NTS1 (IHC-grade phospho-Neurotensin Receptor 1 Antibody)

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Preview: Immunohistochemical identification of Serine409/Serine410 phosphorylation of Neurotensin Receptor 1 in mouse brain.

Preview: Agonist-induced Serine409/Serine410 phosphorylation of the Neurotensin Receptor 1

Immunohistochemical identification of Serine409/Serine410 phosphorylation of Neurotensin Receptor 1 in mouse brain.

Agonist-induced Serine409/Serine410 phosphorylation of the Neurotensin Receptor 1

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Order number: 7TM0309C-IC
Content: 300 µl
Host: Rabbit

Serine409/Serine410 (S409/S410) is major phosphorylation site of the Neurotensin Receptor 1... more

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Serine409/Serine410 (S409/S410) is major phosphorylation site of the Neurotensin Receptor 1 (NTS1). The pS409/pS410-NTS1 antibody detects phosphorylation in response to agonists. S409/S410 phosphorylation is likely to be involved in efficient ligand sequestration by NTS1.

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Alternative Names NTS1, NTR1, Neurotensin Receptor Type 1, Neurotensin... more

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Alternative Names	NTS1, NTR1, Neurotensin Receptor Type 1, Neurotensin Receptor 1
IUPHAR Target ID	309
UniProt ID	P30989
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human NTS1 around the phosphorylation site of Ser409/Ser410.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of Serine409/Serine410 phosphorylation of... more

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Figure 1. Immunohistochemical identification of Serine409/Serine410 phosphorylation of Neurotensin Receptor 1 in mouse brain. Sections were dewaxed, microwaved in citric acid, and incubated with anti-pS409/pS410 NTS1 (non-phospho-Neurotensin receptor 1) antibody (7TM00309C-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Agonist-induced Serine409/Serine410 phosphorylation of the Neurotensin Receptor 1. Upper panel, HEK293 cells stably expressing the Neurotensin receptor 1 (NTS1) were either not exposed or exposed to 100 nM Neurotensin for 30 minutes. Cells were lysed and immunoblotted with the anti-pS409/pS410-NTS1 antibody (7TM0309C-IC) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-NTS1 antibody (7TM0309N) to confirm equal loading of the gel.

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