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- Order number: 7TM0219N-IC
- Content: 100 µl
- Host: Rabbit
The ETA receptor antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human endothelin receptor A. It can be used to detect ETA receptors in Western blots in a phosphorylation-sensitive manner phosphorylation. After agonist activation, ETA is phosphorylated at the carboxyl-terminus. Because the ETA antibody detects the same epitope, it no longer binds to the receptor. It detects selectively ETA receptors that have been hot activated and not phosphorylated. The ETA antibody can also be used to isolate and enrich ETA receptors from cell and tissue lysates. It also detects ETA in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | ETA, EDNRA, Endothelin Receptor A |
| IUPHAR Target ID | 219 |
| UniProt ID | P25101 (human) Q61614 (mouse) P26684 (rat) |
| Western Blot (WB) | 1:1000 |
| Immunocytochemistry (ICC) | 1:200 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide with sequence KNHDQNNHNTDRSSHKDSMN corresponds to amino acids 408-427 in human, mouse and rat ETA |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Immunohistochemical identification of Endothelin Receptor A in mouse heart. Sections were dewaxed, microwaved in citric acid, and incubated with anti-ETA (Endothelin receptor A) antibody (7TM0219N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, ETA receptors were detected at the plasma membrane of cardiomyocytes.
Figure 2. Western blot analysis of Endothelin Receptor A in mouse tissues in vivo. Mouse heart and kidneys were dissected, homogenated and immunoblotted using anti-ETA (Endothelin receptor A) antibody (7TM0219N-IC) at a dilution of 1:1000. Note, absence of ETA receptors in kidney but not in heart.
Figure 3. Immunohistochemical identification of Endothelin Receptor A in human ovarian carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-ETA (Endothelin receptor A) antibody (7TM0219N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, ETA receptors were uniformly detected at the plasma membrane of tumor cells.
Figure 4. Immunocytochemical identification of Endothelin Receptor A in HEK293 cells. HEK293 cells stably expressing the Endothelin Receptor A (ETA) were either not exposed or exposed to 1 μM Endothelin-1 for 30 min and immunocytochemically stained with anti-ETA (Endothelin Receptor A) antibody (7TM0219N-IC) at a dilution of 1:200. Note, ETA receptors were confined to the plasma membrane in untreated cells (0 min). ETA receptors were seen in perinuclear clusters of vesicles after 30 min Endotheline-1 exposure.
Figure 5. Validation of the Endothelin Receptor A in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Endotheline Receptor A (ETA) were lysed and immunoblotted with the phosphorylation-independent anti-ETA antibody (7TM0219N-WB) at a dilution of 1:1000.
