Premium Phosphosite-Specific 7TM Antibodies
Novel Tools for Your GPCR Research
Select Your Country of Delivery below

GPR183 (IHC-grade), Oxysterol Receptor Antibody

NEW
$ 375.00 *

Prices plus VAT plus shipping costs

Ready to ship today,
Delivery time appr. 5-8 days

  • 7TM0081N-IC
  • 100 µl
  • Rabbit
The non-phospho-GPR183 receptor antibody is directed against the distal end of the... more

The non-phospho-GPR183 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human GPR183. It also detects GPR183 in cultured cells and tissue sections by immunohistochemistry. It can be used to detect total GPR183 receptors in Western blots independent of phosphorylation. The GPR183 antibody can also be used to isolate and enrich GPR183 receptors from tissue lysates.

  Alternative Names EBI2, EBV-induced G-protein coupled receptor 2,... more

 

Alternative Names EBI2, EBV-induced G-protein coupled receptor 2, lymphocyte-specific G-protein coupled receptor
IUPHAR Target ID 81
Uniprot ID P32249
Western Blot (WB)  1:1000
Immunohistochemistry (IHC) 1:100
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide corresponding to residues distal end of carboxy-terminal tail of human GPR183.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Immunohistochemical identification of GPR183 Receptor in human spleen. Sections... more

Figure 1. Immunohistochemical identification of GPR183 Receptor in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR183 (non-phospho-GPR183 Receptor) antibody (7TM0081N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, GPR183 receptors were uniformly detected at the plasma membrane of nearly all cells.

Figure 2. Immunohistochemical varification of GPR183 Receptor antibody in human spleen. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-GPR183 (non-phospho-GPR183 Receptor) antibody (7TM0081N-IC). Sections were then incubated with anti-GPR183 (non-phospho-GPR183 Receptor) antibody (7TM0081N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation GPR183 receptors were uniformly detected at the plasma membrane of nearly all cells.

Figure 3. Validation of the GPR183 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR183 Receptor (GPR183) were not exposed or exposed to 100 nM of specific GPR183-Receptor agonist 7α,25-OHC for 5 minutes, lysed and immunoblotted with the phosphorylation-independent anti-GPR183 antibody (7TM0081N-IC) at a dilution of 1:1000.

Read, write and discuss reviews... more
Customer evaluation for "GPR183 (IHC-grade), Oxysterol Receptor Antibody"
Write an evaluation
Evaluations will be activated after verification.

The fields marked with * are required.

Recently viewed