pT347/pT349-GPR183 (phospho-GPR183 Antibody)

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Agonist-induced Threoine347/Serine349 phosphorylation of GPR183

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Order number: 7TM0081B
Content: 100 µl
Host: Rabbit

Threonine347/Threonine349 (T347/T349) is major phosphorylation site of human GPR183 receptor.... more

Threonine347/Threonine349 (T347/T349) is major phosphorylation site of human GPR183 receptor. The pT347/pT349-GPR183 antibody detects phosphorylation in response to agonists. T347/T349 phosphorylation is likely to be involved in efficient ligand sequestration by GPR183.

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Alternative Names EBI2, EBV-induced G-protein coupled receptor 2,... more

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Alternative Names	EBI2, EBV-induced G-protein coupled receptor 2, lymphocyte-specific G-protein coupled receptor
IUPHAR Target ID	81
UniProt ID	P32249
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human GPR183 around the phosphorylation site of Thr347/Thr349.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threoine347/Serine349 phosphorylation of GPR183. Upper... more

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Figure 1. Agonist-induced Threoine347/Serine349 phosphorylation of GPR183. Upper panel, HEK293 cells transiently expressing the human GPR183-Receptor were either not exposed (vehicle) or exposed to 100 nM of specific GPR183-Receptor agonist 7α,25-OHC for 5 minutes. Cells were lysed and immunoblotted with the anti-pT347/pT349-GPR183 antibody (7TM0081B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-GRP183 antibody to confirm equal loading of the gel.

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