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- Order number: 7TM0331N-IC
- Content: 100 µl
- Host: Rabbit
The PTH1 antibody is directed against the distal end of the carboxyl-terminal tail of human Parathyroid Hormone Receptor 1. It can be used to detect total PTH1 receptors in Western blots independent of phosphorylation. The PTH1 antibody can also be used to isolate and enrich PTH1 receptors from cell and tissue lysates. It also detects PTH1 in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | PTH1, Parathyroid Hormone Receptor 1, Parathyroid Hormone-releated Peptide Receptor |
| IUPHAR Target ID | 331 |
| UniProt ID | Q03431 |
| Western Blot (WB) | 1:1000 |
| Immunocytochemistry (ICC) | 1:200 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide with sequence EEASGPERPPALLQEEWETVM corresponds to amino acids 473-493 of human PTH1 |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Immunohistochemical identification of Parathyroid Hormone Receptor 1 in human kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-PTH1 (Parathyroid Hormone Receptor 1) antibody (7TM0331N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, PTH1 receptors were uniformly detected at the basolateral plasma membrane of nearly all tubular cells.
Figure 2. Immunohistochemical identification of Parathyroid Hormone Receptor 1 in human kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-PTH1 (Parathyroid Hormone Receptor 1) antibody (7TM0331N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, PTH1 receptors were uniformly detected at the basolateral plasma membrane of nearly all tubular cells.
Figure 3. Immunohistochemical identification of Parathyroid Hormone Receptor 1 in late distal convoluted tubules and connecting ducts of human kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-PTH1 (Parathyroid Hormone Receptor 1) antibody (7TM0331N-IC) at a dilution of 1:100. Sections were then incubated with anti-rabbit-AlexaFluor488. Note, PTH1 receptors were detected at the plasma membrane of all cells.
Figure 4. Immunohistochemical identification of Parathyroid Hormone Receptor 1 in human prostate carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-PTH1 (Parathyroid Hormone Receptor 1) antibody (7TM0331N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, PTH1 receptors were detected at the plasma membrane of nearly all tumor cells in human prostate carcinoma.
Figure 5. Immunocytochemical identification of Parathyroid Hormone Receptor 1 in transfected HEK293 cells. HEK293 cells stably expressing the Parathyroid Hormone Receptor 1 (PTH1) were either not exposed or exposed to 1 μM PTH 1-34 (first 34 amino acids of human parathyroid hormone) for 30 min and immunocytochemically stained with anti-PTH1 (Parathyroid Hormone Receptor 1 ) antibody (7TM0331N-IC) at a dilution of 1:200. Note, PTH1 receptors were confined to the plasma membrane in untreated cells (0 min). PTH1 receptors were seen in perinuclear clusters of vesicles after 30 min PTH exposure.
Figure 6. Validation of the Parathyroid Hormone Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Parathyroid Hormone Receptor 1 (PTH1) were lysed and immunoblotted with the anti-PTH1 antibody (7TM0331N-IC) at a dilution of 1:1000.
Figure 7. Western blot analysis of Parathyroid Hormone Receptor 1 in membrane preparations from normal human kidney tissue. Samples were seperated on 8% SDS-polyacrylamide gels and blotted onto nitrocellulose membranes. The nitrocellulose membranes were then incubated with anti-PTH1 (Parathyroid Hormone Receptor 1) antibody (7TM0331N-IC) at a dilution of 1:1000 in presence (+) or absence (-) of 10 µg/ml antigen peptide of the antibody. Note, absence PTH1 receptors in presence of antigen peptide.
