FFA4 (IHC-grade), FFA4 Receptor Antibody

Figure 1. Immunohistochemical identification of FFA4 Receptor in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-FFA4 antibody (7TM0127N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, FFA4 receptors were detected at the plasma membrane of cells in human spleen.

Figure 2. Detection of non-phosphorylated human FFA4 Receptor. Flp-In TREx 293 harbouring human FFA4-eYFP induced to express the receptor construct were treated with vehicle or 10 µM TUG-891 for 5 min. Cells were lysed and receptor construct enriched with a GFP-trap, before immunoblotting with anti-non-phospho-FFA4 (7TM0127N-IC) at a dilution of 1:1000 (upper panel) or anti-GFP 1:10,000 to confirm expression and equal loading of the gel (lower panel).