pT347-FFA4 (phospho-FFA4 Antibody)

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Agonist-induced Threonine347 phosphorylation of the FFA Receptor 4

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Order number: 7TM0127A
Content: 100 µl
Host: Rabbit

Threonine347 (T347) is major phosphorylation site of the FFA Receptor 4 (FFA4). The pT347-FFA4... more

Threonine347 (T347) is major phosphorylation site of the FFA Receptor 4 (FFA4). The pT347-FFA4 antibody detects phosphorylation in response to agonists. T347 phosphorylation is likely to be involved in efficient ligand sequestration by FFA4.

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Alternative Names FFAR4, Free Fatty Acid Receptor 4, Omega 3 Fatty Acid... more

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Alternative Names	FFAR4, Free Fatty Acid Receptor 4, Omega 3 Fatty Acid Receptor
IUPHAR Target ID	127
UniProt ID	Q5NUL3
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human FFA4 around the phosphorylation site of Thr347.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine347 phosphorylation of the FFA Receptor 4. Upper panel,... more

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Figure 1. Agonist-induced Threonine347 phosphorylation of the FFA Receptor 4. Upper panel, Flp-In TREx 293 harbouring human FFA4-eYFP induced to express the receptor construct were treated with vehicle or 10 µM TUG-891 for 5 min. Cells were lysed and receptor construct enriched with a GFP-trap, before immunoblotting with the anti-pT347-FFA4 antibody (7TM0127A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-GFP antibody to confirm equal loading of the gel.

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