pS305/pS306 FFA3 (phospho-FFA3 Antibody)

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Agonist-induced Serine305/Serine306 phosphorylation of the FFA Receptor 3

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Order number: 7TM0227A
Content: 100 µl
Host: Rabbit

Serine305/Serine306 (S305/S306) is major phosphorylation site of the FFA Receptor 3 (FFA3). The... more

Serine305/Serine306 (S305/S306) is major phosphorylation site of the FFA Receptor 3 (FFA3). The pS305/pS306-FFA3 antibody detects phosphorylation in response to agonists. S305/S306 phosphorylation is likely to be involved in efficient ligand sequestration by FFA3.

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Alternative Names FFAR3, Free Fatty Acid Receptor 3, GPCR41 IUPHAR... more

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Alternative Names	FFAR3, Free Fatty Acid Receptor 3, GPCR41
IUPHAR Target ID	227
UniProt ID	O14843
Western Blot (WB)	1:500
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human FFA3 around the phosphorylation site of Ser305/Ser306.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine305/Serine306 phosphorylation of the FFA Receptor 3. Upper... more

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Figure 1. Agonist-induced Serine305/Serine306 phosphorylation of the FFA Receptor 3. Upper panel, Flp-In TREx 293 harbouring human FFA3-eYFP induced to express the receptor construct (+ dox) were treated with vehicle, 2mM propanoic acid (C3) or 5µM TUG-1907 for 5 min. Cells were lysed and receptor construct enriched with a GFP-trap, before immunoblotting with the anti-pS305/pS306-FFA3 antibody (7TM0227A) at a dilution of 1:500. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-GFP antibody to confirm equal loading of the gel. TUG-1097 is described in J. Med Chem 63(7):3577-3595

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