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GPR17 (IHC-grade), G Protein-Coupled Receptor 17 Antibody

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  • 7TM0088N-IC
  • 100 µl
  • Rabbit
The GPR17 antibody is directed against the distal end of the carboxyl-terminal tail of mouse,... more

The GPR17 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human GRP17. It can be used to detect total GPR17 receptors in Western blots independent of phosphorylation. The GPR17 antibody can also be used to isolate and enrich GPR17 receptors from brain lysates. It also detects GPR17 in cultured cells and tissue sections by immunohistochemistry.

  Alternative Names GPR17, G Protein-coupled Receptor 17, Uracil... more

 

Alternative Names GPR17, G Protein-coupled Receptor 17, Uracil Nucleotide/Cysteinyl Leukotriene Receptor
IUPHAR Target ID  88
UniProt ID  Q13304 (human) Q6NS65 (mouse) Q09QM4 (rat)
Western Blot (WB)  1:1000
Immunohistochemistry (IHC) 1:100
Species Reactivity   Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide of a part of carboxy-terminal which is identical in human, mouse and rat GPR17
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Immunohistochemical identification of G Protein-coupled Receptor 17 in... more

Figure 1. Immunohistochemical identification of G Protein-coupled Receptor 17 in oligodendrocytes. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR17 (G Protein-coupled Receptor 17) antibody (7TM0088N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR17 receptors were detected at the plasma membrane of cells.

Figure 2. Validation of the G Protein-coupled Receptor 17 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the G Protein-coupled Receptor 17 (GPR17) were lysed and immunoblotted with the phosphorylation-independent anti-GPR17 antibody (7TM0088N-IC) at a dilution of 1:1000.

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