GPR84 (IHC-grade), G Protein-Coupled Receptor 84 Antibody

Figure 1. Immunohistochemical identification of G Protein-coupled Receptor 84 in lymph node. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR84 (G Protein-coupled Receptor 84) antibody (7TM0120N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR84 receptors were detected at the plasma membrane of cells in lymph node.

Figure 2. Immunohistochemical identification of G Protein-coupled Receptor 84 in cerebral cortex. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR84 (G Protein-coupled Receptor 84) antibody (7TM0120N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR84 receptors were detected at the plasma membrane of cells in cerebral cortex.

Figure 3. Validation of the G Protein-coupled Receptor 84 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the G Protein-coupled Receptor 84 (GPR84) were lysed and immunoblotted with the anti-GPR84 antibody (7TM0120N-IC) at a dilution of 1:1000.

Marsango S, Ward RJ, Jenkins L, Butcher AJ, Al Mahmud Z, Dwomoh L, Nagel F, Schulz S, Tikhonova IG, Tobin AB, Milligan G. Selective phosphorylation of threonine residues defines GPR84-arrestin interactions of biased ligands. J Biol Chem. 2022 May;298(5):101932. doi: 10.1016/j.jbc.2022.101932. Epub 2022 Apr 12. PMID: 35427647; PMCID: PMC9118924.