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GPR87 (IHC-grade), G Protein-Coupled Receptor 87 Antibody

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  • 7TM0122N-IC
  • 100 µl
  • Rabbit
The GPR87 antibody is directed against the distal end of the carboxyl-terminal tail of mouse,... more

The GPR87 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human GRP87. It can be used to detect total GPR87 receptors in Western blots independent of phosphorylation. The GPR87 antibody can also be used to isolate and enrich GPR87 receptors from brain lysates. It also detects GPR87 in cultured cells and tissue sections by immunohistochemistry.

  Alternative Names GPR87, G Protein-coupled Receptor 87 IUPHAR Target... more

 

Alternative Names GPR87, G Protein-coupled Receptor 87
IUPHAR Target ID  122
UniProt ID  Q9BY21 (human) Q99MT7 (mouse)
Western Blot (WB)  1:1000
Immunohistochemistry (IHC) 1:100
Species Reactivity   Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide presents distal end of carboxyl-terminal tail which is identical in human and mouse GPR87
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
 Figure 1. Immunohistochemical identification of G Protein-coupled Receptor 87 in kidney.... more

 Figure 1. Immunohistochemical identification of G Protein-coupled Receptor 87 in kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR87 (G Protein-coupled Receptor 87) antibody (7TM0122N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR87 receptors were detected at the plasma membrane of collecting duct cells in kidney.

Figure 2. Validation of the G protein-coupled receptor 87 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the G protein-coupled receptor 87 (GPR87) were lysed and immunoblotted with the phosphorylation-independent anti-GPR87 antibody (7TM0122N-IC) at a dilution of 1:1000.

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