GPR17 (IHC-grade), G Protein-Coupled Receptor 17 Antibody

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Preview: Immunohistochemical identification of G Protein-coupled Receptor 17 in oligodendrocytes.

Preview: Validation of the G Protein-coupled Receptor 17 in transfected HEK293 cells.

Immunohistochemical identification of G Protein-coupled Receptor 17 in oligodendrocytes.

Validation of the G Protein-coupled Receptor 17 in transfected HEK293 cells.

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Order number: 7TM0088N-IC
Content: 100 µl
Host: Rabbit

The GPR17 antibody is directed against the distal end of the carboxyl-terminal tail of mouse,... more

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The GPR17 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human GRP17. It can be used to detect total GPR17 receptors in Western blots independent of phosphorylation. The GPR17 antibody can also be used to isolate and enrich GPR17 receptors from brain lysates. It also detects GPR17 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names GPR17, G Protein-coupled Receptor 17, Uracil... more

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Alternative Names	GPR17, G Protein-coupled Receptor 17, Uracil Nucleotide/Cysteinyl Leukotriene Receptor
IUPHAR Target ID	88
UniProt ID	Q13304 (human) Q6NS65 (mouse) Q09QM4 (rat)
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide of a part of carboxy-terminal which is identical in human, mouse and rat GPR17
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of G Protein-coupled Receptor 17 in... more

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Figure 1. Immunohistochemical identification of G Protein-coupled Receptor 17 in oligodendrocytes. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR17 (G Protein-coupled Receptor 17) antibody (7TM0088N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR17 receptors were detected at the plasma membrane of cells.

Figure 2. Validation of the G Protein-coupled Receptor 17 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the G Protein-coupled Receptor 17 (GPR17) were lysed and immunoblotted with the phosphorylation-independent anti-GPR17 antibody (7TM0088N-IC) at a dilution of 1:1000.