pS329-A2A (phospho-A2A Adenosine Receptor Antibody)

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Agonist-induced Serine329 phosphorylation of the A2A Adenosine Receptor

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Order number: 7TM0019A
Content: 100 µl
Host: Rabbit

Serine329 is a major phosphorylation site of the A2A Adenosine receptor. The pS329-A2A antibody... more

Serine329 is a major phosphorylation site of the A2A Adenosine receptor. The pS329-A2A antibody detects phosphorylation in response to high-efficacy agonists but not after PKC activation. S329 phosphorylation is a key regulator of A2A desensitization, β-arrestin recruitment and internalization.

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Alternative Names A2-AR, Adenosine Receptor A2A IUPHAR Target ID... more

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Alternative Names	A2-AR, Adenosine Receptor A2A
IUPHAR Target ID	19
UniProt ID	P29274
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human A2A around the phosphorylation site of Ser329.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine329 phosphorylation of the A2A Adenosine Receptor. Upper... more

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Figure 1. Agonist-induced Serine329 phosphorylation of the A2A Adenosine Receptor. Upper panel, HEK293 cells stably expressing the A2A Adenosine Receptor (A2A) were either not exposed or exposed to 100 nM NECA for 30 minutes. Cells were lysed and immunoblotted with the anti-pS329-A2A antibody (7TM0019A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-A2A antibody to confirm equal loading of the gel.

Be the first to decipher the A2A phosphorylation barcode and let us know. more

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Be the first to decipher the A2A phosphorylation barcode and let us know.

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