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pS329-A2A (phospho-A2A Adenosine Receptor Antibody)

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Agonist-induced Serine329 phosphorylation of the A2A Adenosine Receptor
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  • 7TM0019A
  • 100 µl
  • Rabbit
Serine329 is a major phosphorylation site of the A2A Adenosine receptor. The pS329-A2A antibody... more

Serine329 is a major phosphorylation site of the A2A Adenosine receptor. The pS329-A2A antibody detects phosphorylation in response to high-efficacy agonists but not after PKC activation. S329 phosphorylation is a key regulator of A2A desensitization, β-arrestin recruitment and internalization.

  Alternative Names A2-AR, Adenosine Receptor A2A IUPHAR Target ID... more

 

Alternative Names A2-AR, Adenosine Receptor A2A
IUPHAR Target ID  19
UniProt ID  P29274
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  -
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human A2A around the phosphorylation site of Ser329.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine329 phosphorylation of the A2A Adenosine Receptor. Upper... more

Figure 1. Agonist-induced Serine329 phosphorylation of the A2A Adenosine Receptor. Upper panel, HEK293 cells stably expressing the A2A Adenosine Receptor (A2A) were either not exposed or exposed to 100 nM NECA for 30 minutes. Cells were lysed and immunoblotted with the anti-pS329-A2A antibody (7TM0019A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-A2A antibody to confirm equal loading of the gel.

Be the first to decipher the A2A phosphorylation barcode and let us know. more

Be the first to decipher the A2A phosphorylation barcode and let us know.

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